Tag Archives: lncrna

Feed Subscription

The dark matter of the cancer genome has been largely neglected

The dark matter of the cancer genome has been largely neglected

Cancer is a disease of the genome caused by oncogene activation and tumor suppressor gene inhibition. Deep sequencing studies including large consortia such as TCGA and ICGC identified numerous tumor-specific mutations not only in protein-coding sequences but also in non-coding ...

Read More »

A comprehensive overview of lncRNA annotation resources

A comprehensive overview of lncRNA annotation resources

Long noncoding RNAs (lncRNAs) are emerging as a class of important regulators participating in various biological functions and disease processes. With the widespread application of next-generation sequencing technologies, large numbers of lncRNAs have been identified, producing plenty of lncRNA annotation ...

Read More »

A Method for Surveying the Long-RNA Landscape of Exosomes

A Method for Surveying the Long-RNA Landscape of Exosomes

The emergence of next-generation sequencing platforms has enabled the discovery and interrogation of RNA from tissues, biofluids, and extracellular vesicles at remarkable levels. Recently, interest has grown in sequencing RNA found in extracellular vesicles called exosomes, because of their role ...

Read More »

Identification of novel long non-coding RNAs using RNA-Seq

Identification of novel long non-coding RNAs using RNA-Seq

Functional characterization of long non-coding RNAs (lncRNAs) and their pathological relevance is still a challenging task. Abnormal expression of a few long non-coding RNAs have been found associated with hepatocellular carcinoma, with potential implications to both improve our understanding of ...

Read More »

Nuclear RNA Isolation and Sequencing

Nuclear RNA Isolation and Sequencing

Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus ...

Read More »

Probing RNA Structure in Cells Using Targeted Structure-Seq

Probing RNA Structure in Cells Using Targeted Structure-Seq

Models for how lncRNAs function depend on thermodynamically stable secondary and higher-order structures that RNAs can form in the context of a cell. Probing accessible RNA bases can provide data to build models of RNA conformation that provide insight into ...

Read More »
Scroll To Top