Tag Archives: next-generation sequencing

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The FDA weighs in on RNA-Seq

The FDA weighs in on RNA-Seq

The rapid advancement of emerging genomics technologies and their application for assessing safety and efficacy of FDA-regulated products require a high standard of reliability and robustness supporting regulatory decision-making in the FDA. To facilitate the regulatory application, the FDA implemented ...

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IsomiR Bank – a research resource for tracking IsomiRs

IsomiR Bank – a research resource for tracking IsomiRs

Next-Generation Sequencing (NGS) technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent differences from their corresponding mature reference sequences, generating multiple variants: the isoforms of miRNAs (isomiRs). These isomiRs mainly originate via the imprecise and alternative cleavage during ...

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Empirical estimation of sequencing error rates using smoothing splines

Empirical estimation of sequencing error rates using smoothing splines

Next-generation sequencing has been used by investigators to address a diverse range of biological problems through, for example, polymorphism and mutation discovery and microRNA profiling. However, compared to conventional sequencing, the error rates for next-generation sequencing are often higher, which ...

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Is Personalized Medicine Here?

Is Personalized Medicine Here?

Clinical application of next-generation sequencing The widespread availability of genomic analysis of tumors in clinical practice has been primarily driven by the advent of next-generation sequencing (NGS). The term “NGS” includes several types of genomic sequencing methodologies, such as targeted ...

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Comprehensive Assessments of RNA-seq by the SEQC Consortium

Comprehensive Assessments of RNA-seq by the SEQC Consortium

Studies on gene expression in response to therapy have led to the discovery of pharmacogenomics biomarkers and advances in precision medicine. Whole transcriptome sequencing (RNA-seq) is an emerging tool for profiling gene expression and has received wide adoption in the ...

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New to RNA-Seq Bioinformatics? Try GeneGazer

New to RNA-Seq Bioinformatics? Try GeneGazer

Next-generation sequencing provides useful information about gene mutations, gene expression, epigenetic modification, microRNA expression, and copy number variations. More and more computing tools have been developed to analyze this large quantity of information. However, to test and find suitable analytical ...

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Measuring the Expression of SINE Retroelements with RNA-Seq

Measuring the Expression of SINE Retroelements with RNA-Seq

The regulation and control of SINE transcription is likely a key process to preserve the physiology and homeostasis of specific tissues and organs. This implies that the analysisof SINE expression is relevant to understand the mechanisms by which these elements ...

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Sequencing Mitochondrial Transcriptomes with RNA-Seq

Sequencing Mitochondrial Transcriptomes with RNA-Seq

Thousands of mitochondrial genomes have been sequenced, but there are comparatively few available mitochondrial transcriptomes. This might soon be changing. High-throughput RNA sequencing (RNA-Seq) techniques have made it fast and cheap to generate massive amounts of mitochondrial transcriptomic data. Here, ...

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