Tag Archives: next-generation sequencing

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Step by Step – RNA-Seq

Step by Step – RNA-Seq

The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, ...

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Extending the sRNAome of Apple by Next-Generation Sequencing

Extending the sRNAome of Apple by Next-Generation Sequencing

The global importance of apple as a fruit crop necessitates investigations into molecular aspects of the processes that influence fruit quality and yield, including plant development, fruit ripening and disease resistance. In order to study and understand biological processes it ...

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Hobbes2 – for fast and accurate alignment of NGS reads

Hobbes2 – for fast and accurate alignment of NGS reads

Next-generation sequencing (NGS) enables rapid production of billions of bases at a relatively low cost. Mapping reads from next-generation sequencers to a given reference genome is an important first step in many sequencing applications. Popular read mappers, such as Bowtie ...

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