Pacific Biosciences of California, Inc. today announced a new version of Sequel® Software (V5.1) and a new polymerase. Combined, these enhancements increase throughput and the overall...
Read More »The Iso-Seq method for transcriptome analysis and genome annotation
The Isoform Sequencing (Iso - Seq™) method employs long reads to sequence transcript isoforms from the 5’ end to their poly - A tails, eliminating the need for transcript reconstruction and inference. These long reads result in complete, unambiguous...
Read More »PacBio full-length transcriptome sequencing of mitochondrial RNAs fundamentally changes the annotation of human mitochondrial genome
In this study, researchers at Nankai University introduced a general framework to use PacBio full-length transcriptome sequencing for the investigation of mitochondrial RNAs. As a result, they produced the first full-length human mitochondrial transcriptome based on the public PacBio data ...
Read More »Recent Publications Demonstrate Rapid Adoption of SMRT Sequencing for Plant and Animal Transcriptome Analysis
A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, ...
Read More »“We see single-cell RNA-seq as the right way to do gene expression analysis”
Some sequencing applications, such as the detection of single nucleotide polymorphisms, can be managed with short-read technology. Other applications, such as the detection of structural variants, may demand long-read technology, and some applications, such as the assembly of a new ...
Read More »LSCplus – a fast solution for improving long read accuracy by short read alignment
The single molecule, real time (SMRT) sequencing technology of Pacific Biosciences enables the acquisition of transcripts from end to end due to its ability to produce extraordinarily long reads (>10 kb). This new method of transcriptome sequencing has been applied ...
Read More »Iso-Seq analysis of SMRT sequencing data produces most detailed maize transcriptome yet
Improvements in maize breeding are required to feed a growing population and will depend on a more complete understanding of global gene expression in the plant. In a new publication from Cold Spring Harbor Laboratory (CSHL), scientists produced the largest ...
Read More »Iso-Seq – Full-length cDNA Sequencing of Alternatively Spliced Isoforms
The majority of human genes are alternatively spliced, making it possible for most genes to generate multiple proteins. Alternative splicing has been shown to play a role in human cancer, muscular dystrophy, Alzheimer’s, and many other diseases. Understanding these diseases ...
Read More »Featured RNA-Seq Job – Sr Research Associate- Sequencing Platforms
Lawrence Berkeley National Laboratory (Berkeley, CA) The Department of Energy (DOE) Joint Genome Institute in Walnut Creek, CA (a division of the Lawrence Berkeley National Lab) is searching for an experienced Research Associate. Under general direction, performs research assignments that ...
Read More »Long-Read Sequencing Offers Better Understanding of Pluripotency
A new review article offers a nice overview of attempts to characterize the transcriptome of human stem cells using RNA-seq, the Iso-Seq™ method, and more. Kin Fai Au and Vittorio Sebastiano, scientists at the University of Iowa and Stanford University, ...
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