Tag Archives: pacific biosciences

Significant Enhancements to Sequel System Enable >99% Accuracy for Single-Molecule Reads up to 15 kb; Throughput Increases by 2- to 4-Fold Pacific Biosciences of California, Inc. a provider of high-quality sequencing of genomes, transcriptomes and epigenomes, today announced major enhancements ...

In cancer cells, genetic errors wreak havoc. Misspelled genes, as well as structural variations—larger-scale rearrangements of DNA that can encompass large chunks of chromosomes—disturb carefully balanced mechanisms that have evolved to regulate cell growth. Genes that are normally silent are...

Pacific Biosciences of California, Inc. today announced a new version of Sequel® Software (V5.1) and a new polymerase. Combined, these enhancements increase throughput and the overall...

The Isoform Sequencing (Iso - Seq™) method employs long reads to sequence transcript isoforms from the 5’ end to their poly - A tails, eliminating the need for transcript reconstruction and inference. These long reads result in complete, unambiguous...

In this study, researchers at Nankai University introduced a general framework to use PacBio full-length transcriptome sequencing for the investigation of mitochondrial RNAs. As a result, they produced the first full-length human mitochondrial transcriptome based on the public PacBio data ...

A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, ...

Some sequencing applications, such as the detection of single nucleotide polymorphisms, can be managed with short-read technology. Other applications, such as the detection of structural variants, may demand long-read technology, and some applications, such as the assembly of a new ...

The single molecule, real time (SMRT) sequencing technology of Pacific Biosciences enables the acquisition of transcripts from end to end due to its ability to produce extraordinarily long reads (>10 kb). This new method of transcriptome sequencing has been applied ...

Improvements in maize breeding are required to feed a growing population and will depend on a more complete understanding of global gene expression in the plant. In a new publication from Cold Spring Harbor Laboratory (CSHL), scientists produced the largest ...

The majority of human genes are alternatively spliced, making it possible for most genes to generate multiple proteins. Alternative splicing has been shown to play a role in human cancer, muscular dystrophy, Alzheimer’s, and many other diseases. Understanding these diseases ...