Tag Archives: post-transcriptional regulation

Leveraging the complementary nature of RNA-Seq and shotgun proteomics data

rna-seq

RNA sequencing (RNA-Seq) and mass spectrometry-based shotgun proteomics are powerful high-throughput technologies for identifying and quantifying RNA transcripts and proteins respectively. With the increasing affordability of these technologies, many projects have started to apply both to the same samples to ...

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Transcription Start Site Associated RNAs (TSSaRNAs) Are Ubiquitous in All Domains of Life

rna-seq

A plethora of non-coding RNAs has been discovered using high-resolution transcriptomics tools, indicating that transcriptional and post-transcriptional regulation is much more complex than previously appreciated. Small RNAs associated with transcription start sites of annotated coding regions (TSSaRNAs) are pervasive in ...

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Comprehensive Analysis of Alternative Splicing by Strand-Specific RNA-Seq

rna-seq

Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific ...

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Ignored biases confound RNA-Seq results

High-throughput RNA sequencing (RNA-seq) continues to provide unparalleled insight into transcriptome complexity. Now the gold standard for assessing global transcript levels, RNA-seq is poised to revolutionize our understanding of transcription and post-transcriptional regulation of RNA. To fully capitalize on the ...

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