Tag Archives: RNA-Seq

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Strategies for transcriptional splice variant detection

Strategies for transcriptional splice variant detection

The advent and improvement of high-throughput sequencing over the past decade leveraged the study of whole genomes and transcriptomes of different organisms at lower costs. In transcriptomics, RNA-Seq expands our capacity to understand gene expression in different tissues and pathologies, ...

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dRNA-Seq – Riboswitch Discovery by Combining RNA-Seq and Genome-Wide Identification of Transcriptional Start Sites

dRNA-Seq – Riboswitch Discovery by Combining RNA-Seq and Genome-Wide Identification of Transcriptional Start Sites

Deep-sequencing technologies applied to RNA have tremendous potential to identify novel transcripts with single-nucleotide resolution. By combining whole-transcript cDNA sequencing (RNA-seq) and genome-wide identification of transcription start sites (dRNA-seq), it is possible to characterize long 5′-untranslated regions potentially endowed with ...

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Disease Biomarker Query from RNA-Seq Data

Disease Biomarker Query from RNA-Seq Data

As a revolutionary way to unveil transcription, RNA-Seq technologies are challenging bioinformatics for its large data volumes and complexities. A large number of computational models have been proposed for differential expression (DE) analysis and normalization from different standing points. However, ...

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QuaCRS – An Integrated RNA-Seq Quality Control Pipeline

QuaCRS – An Integrated RNA-Seq Quality Control Pipeline

QuaCRS (Quality Control for RNA-Seq) is an integrated, simplified quality control (QC) system for RNA-seq data that allows easy execution of several open-source QC tools, aggregation of their output, and the ability to quickly identify quality issues by performing meta-analyses ...

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Most Read Blog Posts – October 2014

Most Read Blog Posts – October 2014

A Comparison of Next Generation Sequencing Technologies for Transcriptome Assembly 1,502 Views Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers 1,471 Views Choosing a next-generation sequencing pipeline 1,284 Views ...

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piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing

piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing

PIWI-interacting RNAs (piRNAs), 23-36 nt small silencing RNAs, repress transposon expression in the metazoan germ line, thereby protecting the genome. Although high-throughput sequencing has made it possible to examine the genome and transcriptome at unprecedented resolution, extracting useful information from ...

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