Tag Archives: RNA-Seq

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Next-Generation Sequencing RNA-Seq Library Construction

Next-Generation Sequencing RNA-Seq Library Construction

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

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piClust: A density based piRNA clustering algorithm

piClust: A density based piRNA clustering algorithm

Piwi-interacting RNAs (piRNAs) are recently discovered, endogenous small non-coding RNAs. piRNAs protect the genome from invasive transposable elements (TE) and sustain integrity of the genome in germ cell lineages. Small RNA-sequencing data can be used to detect piRNA activations in ...

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INsPeCT: INtegrative Platform for Cancer Transcriptomics

INsPeCT: INtegrative Platform for Cancer Transcriptomics

The emergence of transcriptomics, fuelled by high-throughput sequencing technologies, has changed the nature of cancer research and resulted in a massive accumulation of data. Computational analysis, integration, and data visualization are now major bottlenecks in cancer biology and translational research. ...

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Featured RNA-Seq Jobs

Featured RNA-Seq Jobs

Summer Internship at Millennium: The Takeda… Randstad Custom (Cambridge, MA) …mutation calling and copy number estimation (exome sequencing) and differential gene? RNA – Seq ). Run downstream bioinformatics analysis including gene ontology (GO) … sciencecareersite.com (03/20/14) Senior Research Scientist, Computational ...

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RNA-specific temporal dynamic methods still in infancy

RNA-specific temporal dynamic methods still in infancy

Interpreting gene expression profiles often involves statistical analysis of large numbers of differentially expressed genes, isoforms, and alternative splicing events at either static or dynamic spectrums. Reduced sequencing costs have made feasible dense time-series analysis of gene expression using RNA-seq; ...

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Gene Ontology Based Housekeeping Gene Selection for RNA-seq Normalization

Gene Ontology Based Housekeeping Gene Selection for RNA-seq Normalization

RNA-seq analysis provides a powerful tool for revealing relationships between gene expression level and biological function of proteins. In order to identify differentially expressed genes among various RNA-seq datasets obtained from different experimental designs, an appropriate normalization method for calibrating ...

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TRAP – Time-series RNA-seq Analysis Package

TRAP – Time-series RNA-seq Analysis Package

Measuring expression levels of genes at the whole genome level can be useful for many purposes, especially for revealing biological pathways underlying specific phenotype conditions. When gene expression is measured over a time period, we have opportunities to understand how ...

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