Tag Archives: RNA-Seq

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RNA-Seq – revelation of the messengers

Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, researchers from Utrecht University, The Netherlands put ...

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Grants for pilot DNA-Seq and RNA-Seq research projects

Grants for pilot DNA-Seq and RNA-Seq research projects

The Biodesign Institute at ASU’s Personalized Diagnostics Genomics Core together with Illumina invite you to apply for a grant program that offers an opportunity for innovative investigators to pilot DNA-seq and RNA-seq research projects using the Illumina HiSeq2000 DNA sequencer. The ...

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Methods to Study Splicing from RNA-Seq

from figshare – by Eduardo Eyras, Gael P. Alamancos & Eneritz Agirre Graphical representation of methods to study splicing from RNA-Seq. Methods are divided according to whether they perform Mapping, Reconstruction of events or isoforms, Quantification of events and/or isoforms ...

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A new method for stranded whole transcriptome RNA-seq

This report describes an improved protocol to generate stranded, barcoded RNA-seq libraries to capture the whole transcriptome. By optimizing the use of duplex specific nuclease (DSN) to remove ribosomal RNA reads from stranded barcoded libraries, researchers at Indiana University School ...

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SEECER – SEquencing Error CorrEction in Rna-seq data

Sequencing of RNAs (RNA-Seq) has revolutionized the field of transcriptomics, but the reads obtained often contain errors. Read error correction can have a large impact on our ability to accurately assemble transcripts. This is especially true for de novo transcriptome ...

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