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Introduction to RNA Sequencing and Analysis

Introduction to RNA Sequencing and Analysis

RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of ...

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Introduction to RNA Sequencing

Introduction to RNA Sequencing

This lecture explains about the RNA sequencing process and the methods or RNA splicing is explained. It also states the use of RNA sequencing in molecular biology. RNA Sequencing, often known as entire Transcriptome Shotgun Sequencing, is a science that ...

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Quantification of co-transcriptional splicing from RNA-Seq data

Quantification of co-transcriptional splicing from RNA-Seq data

During gene expression, protein-coding transcripts are shaped by multiple processing events: 5′ end capping, pre-mRNA splicing, RNA editing, and 3′ end cleavage and polyadenylation. These events are required to produce mature mRNA, which can be subsequently translated. Nearly all of ...

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Single cell transcriptomics – methods and applications

Single cell transcriptomics – methods and applications

Traditionally, gene expression measurements were performed on “bulk” samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular ...

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Considerations for RNA-Seq Analysis of Circadian Rhythms

Considerations for RNA-Seq Analysis of Circadian Rhythms

Circadian rhythms are daily endogenous oscillations of behavior, metabolism, and physiology. At a molecular level, these oscillations are generated by transcriptional-translational feedback loops composed of core clock genes. In turn, core clock genes drive the rhythmic accumulation of downstream outputs-termed ...

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