Tag Archives: rna sequencing

Feed Subscription

Introduction to RNA Sequencing

Introduction to RNA Sequencing

This lecture explains about the RNA sequencing process and the methods or RNA splicing is explained. It also states the use of RNA sequencing in molecular biology. RNA Sequencing, often known as entire Transcriptome Shotgun Sequencing, is a science that ...

Read More »

Quantification of co-transcriptional splicing from RNA-Seq data

Quantification of co-transcriptional splicing from RNA-Seq data

During gene expression, protein-coding transcripts are shaped by multiple processing events: 5′ end capping, pre-mRNA splicing, RNA editing, and 3′ end cleavage and polyadenylation. These events are required to produce mature mRNA, which can be subsequently translated. Nearly all of ...

Read More »

Single cell transcriptomics – methods and applications

Single cell transcriptomics – methods and applications

Traditionally, gene expression measurements were performed on “bulk” samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular ...

Read More »

Considerations for RNA-Seq Analysis of Circadian Rhythms

Considerations for RNA-Seq Analysis of Circadian Rhythms

Circadian rhythms are daily endogenous oscillations of behavior, metabolism, and physiology. At a molecular level, these oscillations are generated by transcriptional-translational feedback loops composed of core clock genes. In turn, core clock genes drive the rhythmic accumulation of downstream outputs-termed ...

Read More »

The landscape of long noncoding RNAs in the human transcriptome

The landscape of long noncoding RNAs in the human transcriptome

Long noncoding RNAs (lncRNAs) are emerging as important regulators of tissue physiology and disease processes including cancer. To delineate genome-wide lncRNA expression, researchers at the University of Michigan curated 7,256 RNA sequencing (RNA-seq) libraries from tumors, normal tissues and cell ...

Read More »
Scroll To Top