Tag Archives: rna structure

The function of many noncoding RNAs (ncRNAs) depend upon their secondary structures. Over the last decades, several methodologies have been...

October 2–6 2017 CEITEC MU, University Campus Bohunice Kamenice 5, Brno, Czech Republic The objective of the summer school is to provide participants with an overview on the most up-to-date topics in RNA research. The lectures from leading experts in ...

The rapidly increasing number of discovered non-coding RNAs makes the understanding of their structure a key feature toward a deeper comprehension of gene expression regulation. Various enzymatic- and chemically- based approaches have been recently developed to allow whole-genome studies of ...

Models for how lncRNAs function depend on thermodynamically stable secondary and higher-order structures that RNAs can form in the context of a cell. Probing accessible RNA bases can provide data to build models of RNA conformation that provide insight into ...

The combination of Reverse Transcription (RT) and high-throughput sequencing has emerged as a powerful combination to detect modified nucleotides in RNA via analysis of either abortive RT-products or of the incorporation of mismatched dNTPs into cDNA. Here researchers from the ...

from Nature Biotechnology by Eric Westhof Solving the three-dimensional structure of an RNA molecule means laborious study by X-ray crystallography or nuclear magnetic resonance spectroscopy. However, faster complementary methods are on the horizon, many involving deep sequencing and sophisticated computational ...

A key aspect of RNA secondary structure prediction is the identification of novel functional elements. This is a challenging task because these elements typically are embedded in longer transcripts where the borders between the element and flanking regions have to ...

Transcriptome-wide RNA structure probing coupled with NGS yields the RNA structurome. RNA structurome data tremendously improve prediction of in vivo RNA structure. RNA structurome data uncover relationships between RNA structure and gene regulation. RNA folds into intricate structures that enable ...

Temperature is one of the decisive signals that a mammalian pathogen has entered its warm-blooded host. Among the many ways to register temperature changes, bacteria often use temperature-modulated structures in the untranslated region of mRNAs. In this article, the authors ...

by Xiaole Shirley Liu RNA-seq Experiments Analysis: read mapping, expression index, isoform inference, differential expression RNA structure prediction Covariance model Base-pair maximization Free energy method