Tag Archives: rpkm

You’ve been doing your RNA-Seq all wrong

rna-seq

In recent years, RNA-seq is emerging as a powerful technology in estimation of gene and/or transcript expression, and RPKM (Reads Per Kilobase per Million reads) is widely used to represent the relative abundance of mRNAs for a gene. In general, ...

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RPKM, FPKM and TPM, clearly explained

rna-seq

from StatQuest It used to be when you did RNA-seq, you reported your results in RPKM (Reads Per Kilobase Million) or FPKM (Fragments Per Kilobase Million). However, TPM (Transcripts Per Kilobase Million) is now becoming quite popular. Since there seems ...

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ReadXplorer – Visualization and Analysis of Mapped Sequences

rna-seq

Fast algorithms and well-arranged visualizations are required for the comprehensive analysis of the ever-growing size of genomic and transcriptomic next generation sequencing (NGS) data. ReadXplorer is a software offering straightforward visualization and extensive analysis functions for genomic and transcriptomic DNA ...

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Optimal Calculation of RNA-Seq Fold-Change Values

rna-seq

Biologists often use RNA-Sequencing (RNA-Seq) to identify a limited number of genes for subsequent validation, and one important factor for candidate gene selection is the fold-change in expression between two groups. However, RNA-Seq produces a wide range of read counts ...

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