Tag Archives: The Cancer Genome Atlas

Gene Expression’s Big Rethink

rna-seq

Genetic Engineering News by Richard Stein – Two technologies used to interrogate gene expression, RNA-Seq and microarray analysis, often return strongly correlated results. These technologies, however, have not been evaluated for their concordance at the isoform level. To understand the ...

Read More »

The Pitfalls of RNA-Seq Methodology

rna-seq

TP53 undergoes multiple RNA-splicing events, resulting in at least nine mRNA transcripts encoding at least 12 functionally different protein isoforms. Antibodies specific to p53 protein isoforms have proven difficult to develop, thus researchers must rely on the transcript information to ...

Read More »