Tag Archives: transcriptome assembly

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Analysis of RNA-Seq Data Using TopHat and Cufflinks

Analysis of RNA-Seq Data Using TopHat and Cufflinks

The recent advances in high throughput RNA sequencing (RNA-Seq) have generated huge amounts of data in a very short span of time for a single sample. These data have required the parallel advancement of computing tools to organize and interpret ...

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SATRAP – SOLiD Assembler TRAnslation Program

SATRAP – SOLiD Assembler TRAnslation Program

SOLiD DNA sequences are typically analyzed using a reference genome, while they are not recommended for de novo assembly of genomes or transcriptomes. This is mainly due to the difficulty in translating the SOLiD color-space data into normal base-space sequences. ...

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A comparative study of RNA-seq analysis strategies

A comparative study of RNA-seq analysis strategies

Three principal approaches have been proposed for inferring the set of transcripts expressed in RNA samples using RNA-seq. The simplest approach uses curated annotations, which assumes the transcripts in a sample are a subset of the transcripts listed in a ...

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CLASS – Splice Variant Annotation from RNA-Seq Reads

CLASS – Splice Variant Annotation from RNA-Seq Reads

Next generation sequencing of cellular RNA is making it possible to characterize genes and alternative splicing in unprecedented detail. However, designing bioinformatics tools to capture splicing variation accurately has proven difficult. Current programs find major isoforms of a gene but ...

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Bayesembler – bayesian transcriptome assembly

Bayesembler – bayesian transcriptome assembly

RNA-seq allows for simultaneous transcript discovery and quantification, but reconstructing complete transcripts from such data remains difficult. Here, researchers from the University of Copenhagen introduce the Bayesembler, a novel probabilistic method for transcriptome assembly built on a Bayesian model of ...

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