Tag Archives: transcriptome assembly

Feed Subscription

Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes

Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes

High-throughput RNA sequencing (RNA-seq) provides a comprehensive picture of the transcriptome, including the identity, structure, quantity, and variability of expressed transcripts in cells, through the assembly of sequenced short RNA-seq reads. Although the reference-based approach guarantees the high quality of ...

Read More »

Analysis of RNA-Seq Data Using TopHat and Cufflinks

Analysis of RNA-Seq Data Using TopHat and Cufflinks

The recent advances in high throughput RNA sequencing (RNA-Seq) have generated huge amounts of data in a very short span of time for a single sample. These data have required the parallel advancement of computing tools to organize and interpret ...

Read More »

SATRAP – SOLiD Assembler TRAnslation Program

SATRAP – SOLiD Assembler TRAnslation Program

SOLiD DNA sequences are typically analyzed using a reference genome, while they are not recommended for de novo assembly of genomes or transcriptomes. This is mainly due to the difficulty in translating the SOLiD color-space data into normal base-space sequences. ...

Read More »

A comparative study of RNA-seq analysis strategies

A comparative study of RNA-seq analysis strategies

Three principal approaches have been proposed for inferring the set of transcripts expressed in RNA samples using RNA-seq. The simplest approach uses curated annotations, which assumes the transcripts in a sample are a subset of the transcripts listed in a ...

Read More »
Scroll To Top