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SCOPE++ : Sequence Classification Of homoPolymer Emissions

SCOPE++ : Sequence Classification Of homoPolymer Emissions

mRNA polyadenylation, the addition of a poly(A) tail to the 3′-end of pre-mRNA, is a process critical to gene expression and regulation in eukaryotes. To understand the molecular mechanisms governing polyadenylation and other relevant biological processes, it is important to ...

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Going deep to improve maize transcriptome

Going deep to improve maize transcriptome

from Phys.org A team of researchers from the U.S. Department of Energy Joint Genome Institute (DOE JGI), the University of California, Berkeley, and the Great Lakes Bioenergy Research Center generated an ultra-deep, high quality transcriptome–the fraction of the genome that ...

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Next-Generation Sequencing RNA-Seq Library Construction

Next-Generation Sequencing RNA-Seq Library Construction

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

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Transcriptome analysis of alternative splicing events

Transcriptome analysis of alternative splicing events

Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, researchers at the Chinese Academy of Sciences used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. They found ...

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Shark Transcriptome Less Fishy, More Human Than Expected

Shark Transcriptome Less Fishy, More Human Than Expected

from genetic Engineering News Researchers primarily interested in conserving the great white shark undertook a gene-level analysis of the remarkable creature, which remains mysterious even though it continues to hold the public’s imagination as firmly as its jaws seize prey. ...

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Atypical RNAs in the coelacanth transcriptome

Atypical RNAs in the coelacanth transcriptome

Circular and apparently trans-spliced RNAs have recently been reported as abundant types of transcripts in mammalian transcriptome data. Both types of non-colinear RNAs are also abundant in RNA-seq of different tissue from both the African and the Indonesian coelacanth. A ...

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Parallel deep transcriptome and proteome analysis

Parallel deep transcriptome and proteome analysis

Sensitivity and throughput of transcriptomic and proteomic technologies have advanced tremendously in recent years. With the use of deep sequencing of RNA samples (RNA-seq) and mass spectrometry technology for protein identification and quantitation, it is now feasible to compare gene ...

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