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SCOPE++ : Sequence Classification Of homoPolymer Emissions

SCOPE++ : Sequence Classification Of homoPolymer Emissions

mRNA polyadenylation, the addition of a poly(A) tail to the 3′-end of pre-mRNA, is a process critical to gene expression and regulation in eukaryotes. To understand the molecular mechanisms governing polyadenylation and other relevant biological processes, it is important to ...

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Going deep to improve maize transcriptome

Going deep to improve maize transcriptome

from Phys.org A team of researchers from the U.S. Department of Energy Joint Genome Institute (DOE JGI), the University of California, Berkeley, and the Great Lakes Bioenergy Research Center generated an ultra-deep, high quality transcriptome–the fraction of the genome that ...

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Next-Generation Sequencing RNA-Seq Library Construction

Next-Generation Sequencing RNA-Seq Library Construction

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

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Transcriptome analysis of alternative splicing events

Transcriptome analysis of alternative splicing events

Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, researchers at the Chinese Academy of Sciences used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. They found ...

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Shark Transcriptome Less Fishy, More Human Than Expected

Shark Transcriptome Less Fishy, More Human Than Expected

from genetic Engineering News Researchers primarily interested in conserving the great white shark undertook a gene-level analysis of the remarkable creature, which remains mysterious even though it continues to hold the public’s imagination as firmly as its jaws seize prey. ...

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