Tag Archives: transcriptome

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An optimized protocol for RNA-Seq on the Ion Proton sequencer

An optimized protocol for RNA-Seq on the Ion Proton sequencer

Previous studies compared running cost, time and other performance measures of popular sequencing platforms. However, comprehensive assessment of library construction and analysis protocols for Proton sequencing platform remains unexplored. Unlike Illumina sequencing platforms, Proton reads are heterogeneous in length and ...

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Optimizing RNA-Seq Mapping with STAR

Optimizing RNA-Seq Mapping with STAR

Sequencing of transcribed RNA molecules (RNA-seq) is an invaluable tool for studying cell transcriptomes at high resolution and depth. RNA-seq datasets typically consist of tens to hundreds of millions of relatively short (30–200 nt) sequence fragments of the original RNA ...

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Deciphering the Cancer Transcriptome

Deciphering the Cancer Transcriptome

from Genetic Engineering News by Richard A. Stein – In recent years, scientists have adopted a gene-centric view of cancer, a tendency to see each malignant transformation as the consequence of alterations in a discrete number of genes or pathways. ...

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Penn Researchers Illuminate “Dark Side” of the Transcriptome

Penn Researchers Illuminate “Dark Side” of the Transcriptome

New approach to quantifying gene “read-outs” reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases PHILADELPHIA— A new way of mapping the “transcriptome” — the collection of RNA read-outs that are expressed by a cell’s active ...

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RNA sequencing of the nephron transcriptome: a technical note

RNA sequencing of the nephron transcriptome: a technical note

To understand the functions of the kidney, the transcriptome of each part of the nephron needs to be profiled using a highly sensitive and unbiased tool. RNA sequencing (RNA-seq) has revolutionized transcriptomic research, enabling researchers to define transcription activity and ...

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