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Next-Generation Sequencing RNA-Seq Library Construction

Next-Generation Sequencing RNA-Seq Library Construction

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

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Transcriptome analysis of alternative splicing events

Transcriptome analysis of alternative splicing events

Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, researchers at the Chinese Academy of Sciences used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. They found ...

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Shark Transcriptome Less Fishy, More Human Than Expected

Shark Transcriptome Less Fishy, More Human Than Expected

from genetic Engineering News Researchers primarily interested in conserving the great white shark undertook a gene-level analysis of the remarkable creature, which remains mysterious even though it continues to hold the public’s imagination as firmly as its jaws seize prey. ...

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Atypical RNAs in the coelacanth transcriptome

Atypical RNAs in the coelacanth transcriptome

Circular and apparently trans-spliced RNAs have recently been reported as abundant types of transcripts in mammalian transcriptome data. Both types of non-colinear RNAs are also abundant in RNA-seq of different tissue from both the African and the Indonesian coelacanth. A ...

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Parallel deep transcriptome and proteome analysis

Parallel deep transcriptome and proteome analysis

Sensitivity and throughput of transcriptomic and proteomic technologies have advanced tremendously in recent years. With the use of deep sequencing of RNA samples (RNA-seq) and mass spectrometry technology for protein identification and quantitation, it is now feasible to compare gene ...

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To Pool or not to Pool

To Pool or not to Pool

For non-model organisms, genome-wide information that describes functionally relevant variation may be obtained by RNA-Seq following de novo transcriptome assembly. While sequencing has become relatively inexpensive, the preparation of a large number of sequencing libraries remains prohibitively expensive for population ...

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Presentations from the inaugural RNA-Seq 2013 meeting

Presentations from the inaugural RNA-Seq 2013 meeting

Developing Novel Tools for Transcriptome Analysis to Optimally Utilize RNA-Seq Data. Mark Gerstein, Professor, Biomedical Informatics, Yale University. Mark played an integral role in the development of the first RNA-Seq tools. DEFINITELY worth a read. Characterization of Explant Models Using ...

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