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Scrimer – designing primers from transcriptome data

Scrimer – designing primers from transcriptome data

With the rise of next-generation sequencing methods it has become increasingly possible to obtain genome-wide sequence data even for non-model species. Such data are often used for the development of single nucleotide polymorphism (SNP) markers, which can subsequently be screened ...

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Stranded Whole Transcriptome RNA-Seq for All RNA Types

Stranded Whole Transcriptome RNA-Seq for All RNA Types

Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA (Piwi-interacting RNA), snoRNA (small nucleolar RNA), lincRNA (large non-coding intergenic RNA), SRP RNA (signal recognition ...

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REDItools – Uncovering RNA Editing Sites in Long Non-Coding RNAs

REDItools – Uncovering RNA Editing Sites in Long Non-Coding RNAs

RNA editing is an important co/post-transcriptional molecular process able to modify RNAs by nucleotide insertions/deletions or substitutions. In human, the most common RNA editing event involves the deamination of adenosine (A) into inosine (I) through the adenosine deaminase acting on ...

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AtCAST3.0 Update – A web-based tool for analysis of transcriptome data by searching similarities in gene expression profiles

AtCAST3.0 Update – A web-based tool for analysis of transcriptome data by searching similarities in gene expression profiles

In transcriptome experiments, the experimental conditions (e.g., mutants and/or treatments) cause transcriptional changes. Identifying experimental conditions that induce similar or opposite transcriptional changes can be used to identify experimental conditions that affect the same biological process. AtCAST is a web-based ...

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Microarray Not Fade Away

Microarray Not Fade Away

Yes, many are attracted to next-generation sequencing, but loyalty to chip-based phenotyping has its rewards, particularly in screens of large sample sets. from Genetic Engineering News – by Lisa Heiden The microarray, the legacy technology for measuring gene expression, will ...

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