Tag Archives: transcriptome

RNA-Seq and Socioeconomics

rna-seq

The correlation between low socioeconomic status (SES) and poor health outcome or higher risk of disease has been consistently reported by many epidemiological studies across various race/ancestry groups. However, the biological mechanisms...

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Arkas – Rapid reproducible RNA-Seq analysis

rna-seq

The recently introduced Kallisto pseudoaligner has radically simplified the quantification of transcripts in RNA-sequencing experiments.  Researchers from the Keck School of Medicine of USC offer cloud-scale RNAseq pipelines Arkas-Quantification, which deploys Kallisto for parallel cloud computations, and Arkas-Analysis, which annotates ...

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Unleash the power within RNA-seq

RNA-sequencing (RNA-seq) is an essential technique for transcriptome studies, hundreds of analysis tools have been developed since it was debuted. Although recent efforts have attempted to assess the latest available tools, they have not evaluated the analysis workflows comprehensively to ...

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Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

rna-seq

Obtaining high quality RNA from complex biological tissues, such as the brain, is needed for establishing high-fidelity cell-type specific transcriptomes. Although combining genetic labeling techniques with laser capture microdissection (LCM) is generally sufficient, concerns over RNA degradation and limited yields ...

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Large discordance between transcriptome and proteome

rna-seq

During in vitro differentiation, pluripotent stem cells undergo extensive remodeling of their gene expression. While studied extensively at the transcriptome level, much less is known about protein dynamics, which can differ significantly from their mRNA counterparts. Here, researchers from Leiden ...

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