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Buliding a Human from Transcriptional Profiles

Buliding a Human from Transcriptional Profiles

The manual for human development is written in the genetic code of our DNA. Our genes encode the instructions to make specific types of molecules that are needed to either build the human body or to control its day-to-day operations. ...

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Sift, Sort, Seize Transcriptomic Gold

Sift, Sort, Seize Transcriptomic Gold

from Genetic Engineering News – by Richard A. Stein – The transcriptome is less a tranquil pool than a turbulent stream that keeps shifting its course and adopting new patterns of gene expression. To survey this stream, one must repeatedly ...

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An optimized protocol for RNA-Seq on the Ion Proton sequencer

An optimized protocol for RNA-Seq on the Ion Proton sequencer

Previous studies compared running cost, time and other performance measures of popular sequencing platforms. However, comprehensive assessment of library construction and analysis protocols for Proton sequencing platform remains unexplored. Unlike Illumina sequencing platforms, Proton reads are heterogeneous in length and ...

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Optimizing RNA-Seq Mapping with STAR

Optimizing RNA-Seq Mapping with STAR

Sequencing of transcribed RNA molecules (RNA-seq) is an invaluable tool for studying cell transcriptomes at high resolution and depth. RNA-seq datasets typically consist of tens to hundreds of millions of relatively short (30–200 nt) sequence fragments of the original RNA ...

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