Tag Archives: tRNA

Accurate mapping of tRNA reads

rna-seq

Many repetitive DNA elements are transcribed at appreciable expression levels. Mapping the corresponding RNA sequencing reads back to a reference genome is notoriously difficult and error-prone task, however. This is in particular true if chemical modifications introduce systematic mismatches, while ...

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SHOT-RNAs invisible in RNA-Seq data

Since their discovery in the 1950s, transfer RNAs (tRNAs) have been best known for their role in helping the cell make proteins from messenger RNA templates. However, recent studies have led to a previously-unsuspected concept that tRNAs are not always ...

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Sequencing tRNA

rna-seq

High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. Widely used RNA-seq methods start with adaptor ligation and cDNA synthesis of biological RNA samples followed by PCR amplification to generate sequencing libraries. These standard methods work well for ...

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