Tag Archives: Wellcome Trust Sanger Institute

Power analysis of single-cell RNA-sequencing experiments

Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and providing insights into developmental processes and transcriptional stochasticity. A key question is how the variety of available protocols ...

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Power Analysis of Single Cell RNA Sequencing Experiments

rna-seq

High-throughput single cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, and has revealed new cell types, and new insights into developmental process and stochasticity in gene expression. There are now several published ...

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SC3 – consensus clustering of single-cell RNA-Seq data

Using single-cell RNA-seq (scRNA-seq), the full transcriptome of individual cells can be acquired, enabling a quantitative cell-type characterisation based on expression profiles. However, due to the large variability in gene expression, identifying cell types based on the transcriptome remains challenging. ...

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Fine-mapping cellular QTLs with RASQUAL and ATAC-seq

When cellular traits are measured using high-throughput DNA sequencing, quantitative trait loci (QTLs) manifest as fragment count differences between individuals and allelic differences within individuals. Researchers from the Wellcome Trust Sanger Institute have developed RASQUAL (Robust Allele-Specific Quantitation and Quality Control), ...

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