MicroRNAs are indispensable players in the regulation of a broad range of biological processes. Here, researchers from Zhejiang Sci-Tech University, China report the first deep sequencing of the whitespotted bamboo shark (Chiloscyllium plagiosum) liver. They mapped 91 miRNAs in the Callorhinchus milii genome that have previously been described in the Danio rerio, Fugu rubripes, Oryzias latipes, Xenopus laevis, Xenopus tropicalis, Homo sapiens, and Mus musculus. In addition, 156 new putative candidate (PC) Chiloscyllium plagiosum miRNAs were identified. From these 247 miRNAs, 39 miRNAs clusters were identified, and the expression of these clustered miRNAs were observed to vary significantly. A total of 7 candidate miRNAs were selected for expression confirmation by stem-loop RT-PCR.
This study resulted in the addition of a significant number of novel miRNAs sequences to GenBank and laid the foundation for further understanding of the function of miRNAs in the regulation of Chiloscyllium plagiosum liver development.
- Zhang J, Liu Y, Zhang X, Pan J, Nie Z, Zhang W, Yu W, Chen J, Liu L, Li J, Zhang Y, Guo J, Wu W, Zhu H, Lv Z. (2013) The identification of microRNAs in the whitespotted bamboo shark (Chiloscyllium plagiosum) liver by Illumina sequencing. Gene [Epub ahead of print]. [abstract]