RNA-seq produces millions of sequences from complex RNA samples. With this powerful approach, you can:
- Measure gene expression.
- Discover and annotate complete transcripts.
- Characterize alternative splicing and polyadenylation.
The RNA-seqlopedia provides an overview of RNA-seq and of the choices necessary to carry out a successful RNA-seq experiment.
A typical RNA-seq experiment consists of the following steps:
Each of the five chapters below are dedicated to one of these steps.