Filed Under Transcriptome Sequenced
Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here, the International Barley Genome Sequencing Consortium presents an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. They developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Their data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
- The International Barley Genome Sequencing Consortium. (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature [Epub ahead of print]. [article]
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- differential rna sequencing
- barley rna-seq
- DNA sequence assembly