Transcriptome Sequencing – RFQ

 US Army


Solicitation Number: W81EWF20741883
Agency: Department of the Army
Office: U.S. Army Corps of Engineers
Location: USACE ITL, GSL, EL, CHL, Vicksburg

The following are needed to deliver the sequenced and annotated transcriptome required for the successful execution of the ERDC EL’s research effort:

a. >500bp nucleic acid sequence average read lengths (GS-FLX+ Sequencer is the only machine to achieve required read lengths in a high-throughput format (see requirement b)).
b.   1.4 billion bases total read coverage (2 full plate runs on GS-FLX).
c.   Quality filtering of raw nucleic acid sequence data.
d.   Nucleic acid sequence clustering.
e.   Nucleic acid sequence assembly into contiguous sequences
f.   Contiguous sequence matched against GenBank nucleic acid sequence database.
g.   Sequences translated to amino acid code and matched to protein orthologs.
h.   Functional annotation including gene ontology (GO) associations and Kyoto Encyclopedia of Genes and Genomes (KEGG) database matches to canonical pathways.
i.   Prompt, on-demand bioinformatics support for one year

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