Direct RNA sequencing of genes with polymorphisms can provide quantitative estimates of maternal and paternal alleles’ expression, not just the total gene expression. Narayanan Raghupathy, Ph.D., a postdoctoral fellow working in Gary Churchill’s laboratory at The Jackson Laboratory, will discuss the challenges of estimating gene and allele-specific expression from RNA-seq data.
Raghupathy will present the computational tools, Seqnature and EMASE, used to build personalized genomes and quantify gene and allele-specific expression. He will also present results from F1 hybrid mouse studies to understand the battle of the sexes. His talk will highlight the application of these tools for human and other types of sequencing data.