Upcoming Webinar – Linking T Cell Clonotype to Phenotype with TCR Single-Cell RNA Seq

Mike Stubbington, PhD
Senior Staff Scientist, Wellcome Trust Sanger Institute

Tapio Lonnberg, PhD
Postdoctoral Fellow, EMBL-EBI, Visiting Scientist at Wellcome Trust Sanger Institute

DATE:  May 26, 2016
TIME:  8am pacific time, 11am eastern time

Join us live and learn about a breakthrough in TCR sequencing using single-cell analysis.

T cells recognize specific antigens via T cell receptors (TCRs), an extremely diverse set of cell surface proteins produced by the rearrangement of germline V(D)J gene segments during T cell development. In the April 2016 issue of Nature Methods, scientists at the European Molecular Biology Laboratory European Bioinformatics Institute (EMBL-EBI) and the Wellcome Trust Sanger Institute (WTSI) published a new approach for single-cell TCR sequencing—an approach that can bring new insights about the response to immunotherapies and cancer vaccines, autoimmune disease, allergy, and vaccine design and efficacy.

Using Fluidigm® single-cell whole transcriptome RNA sequencing on the C1™ system and a novel bioinformatics tool, these researchers reconstructed the full-length, paired TCR sequences from mouse T cells. In this webinar, Michael Stubbington will present TraCeR, a novel bioinformatics tool developed to identify the single-cell TCR sequences, along with graphing functions enabling correlation of clonotype and phenotype for each T cell. Tapio Lönnberg will demonstrate the power of TraCeR analysis to elucidate the cell fate decision process as activated T cell clones differentiate in parallel with both Th1 and Tfh effector cell subsets.

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