Video Protocol – Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

The authors describe a protocol for the Illumina Genome Analyzer II platform for mRNA-Seq sequencing for library preparation that avoids significant PCR amplification and requires only 10 nanograms of total RNA. While this protocol has been described previously and validated for single-end sequencing, where it was shown to produce directional libraries without introducing significant amplification bias, here they validate it further for use as a paired end protocol.

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library preparation

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