10x Genomics, Inc., a company focused on accelerating genomic discovery, today announced that BioLegend and Immudex will be the first 10x Compatible Partners providing products for its new single cell Feature Barcoding Technology, which was revealed at the 2018 Advances in Genome Biology and Technology meeting in February this year. The resulting product innovations, available at the end of 2018, will enable simultaneous gene and protein expression of the same cell as well as cellular characterizations and mapping responses. The newly developed applications have potential to accelerate disease research and drug discovery in oncology, neurology, infections disease, and immunology in addition to enhancing cell atlas research initiatives.
“We are proud to partner with 10x Genomics to commercialize this groundbreaking method for multi-omic single cell analysis and to offer a complete solution for researchers,” stated Gene Lay, chief executive officer of BioLegend. “Such high resolution and complex information can be used to more deeply characterize highly heterogeneous cellular populations and to further identify rare and important cells within those populations. These insights can potentially lead to the identification of novel molecular therapeutic targets and can accelerate drug discovery in immuno-oncology, autoimmunity, neurodegeneration, and other areas of human disease.”
The co-development partnership with BioLegend will result in the first commercially offered ready to use solution for simultaneous measurement of highly-multiplexed protein and unbiased gene expression from single cells, similar to previously reported academic methods, CITE-seq and REAP-seq. The new products offered by BioLegend, TotalSeq B and TotalSeq C, will be fully compatible with 10x Genomics’ Chromium Single Cell Gene Expression Solution or Single Cell Immune Profiling Solution with Feature Barcoding Technology.
The additional partnership with Immudex leverages its proprietary Dextramer® technology to interrogate antigen-binding specificity alongside the transcriptome and repertoire readouts at single cell resolution. Using the 10x Chromium Single Cell Immune Profiling Solution with new Feature Barcoding Technology and Immudex DNA barcoded MHC-peptide multimers, researchers will be able to link full-length, paired TCR alpha and beta chain sequences and transcriptional profiles to the identity of their target antigens in the same cells with high specificity and sensitivity.
“By combining our proprietary DNA barcoded Dextramer® technology with 10x Genomics Chromium System and Solutions, we now have the ability to directly interrogate the TCR of the antigen-specific T Cells via MHC-peptide binding along with simultaneous analysis of expressed genes and cell surface phenotype for an entire sample at the single cell level, which has not been possible with existing technologies,” says Liselotte Brix, chief scientific officer of Immudex.
“Through our 10x Compatible Product Partnership Program we are able to rapidly extend our expanding portfolio of applications to our customers worldwide,” stated Brad Crutchfield, chief commercial officer of 10x Genomics. “We seek out leading companies that complement our product portfolio, establishing partnerships that provide our customers with verified solutions. These partnerships are especially important as we continue to grow at a rapid pace.”
To learn more about Single Cell Feature Barcoding Technology, register for these upcoming webinars:
- August 2, 2018 – “Single Cell Feature Barcoding Technology: Simultaneously Examine Gene Expression and Protein Abundance or CRISPR-Mediated Perturbations in the Same Cell.”
- August 9, 2018 – “Single Cell Feature Barcoding Technology: Cellular Characterization and Mapping of Individual T-Cell Responses at Scale.”
More information about CITE-seq and REAP-seq can be found in the following publications:
- [CITE-seq] Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 http://dx.doi.org/10.1038/nmeth.4380 (2017).
- [REAP-seq] Peterson, V.M. et al. Multiplexed quantification of proteins and transcripts in single cells. Nat. Biotechnol. http://dx.doi.org/10.1038/nbt.3973 (2017).
Source – BusinessWire