Search Results for: isomirs

QuickMIRSeq: a pipeline for quick and accurate quantification of both known miRNAs and isomiRs

http://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-017-1601-4 Genome-wide miRNA expression data can be used to study miRNA dysregulation comprehensively. Although many open-source tools for microRNA (miRNA)-seq data analyses are available, challenges remain in accurate miRNA quantification from large-scale miRNA-seq dataset. We implemented a pipeline called QuickMIRSeq ...

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IsomiR Bank – a research resource for tracking IsomiRs

rna-seq

Next-Generation Sequencing (NGS) technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent differences from their corresponding mature reference sequences, generating multiple variants: the isoforms of miRNAs (isomiRs). These isomiRs mainly originate via the imprecise and alternative cleavage during ...

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An evaluation of high-throughput isomiR identification tools

MicroRNAs carry out post-transcriptional gene regulation in animals by binding to the 3′ untranslated regions of mRNAs, causing their degradation or translational repression. MicroRNAs influence many biological functions, and dysregulation can therefore disrupt development or even cause death. High-throughput sequencing ...

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