Single-cell RNA sequencing has led to unprecedented levels of data complexity. Although several computational platforms are available, performing data analyses for multiple datasets remains a significant challenge. University of Oxford researchers provide a comprehensive analytical protocol to interrogate multiple datasets on SingCellaR, an analysis package in R. This tool can be applied to general single-cell transcriptome analyses. The researchers demonstrate steps for data analyses and visualization using bespoke pipelines, in conjunction with existing analysis tools to study human hematopoietic stem and progenitor cells.
A comprehensive analytical protocol to interrogate multiple single-cell RNA-seq datasets with SingCellaR
Wang G, Wen WX, Mead AJ, Roy A, Psaila B, Thongjuea S. (2022) Processing single-cell RNA-seq datasets using SingCellaR. STAR Protoc 3(2):101266. [article]