Advances in non-coding RNA sequencing

Non-coding RNAs (ncRNAs) comprise a set of abundant and functionally diverse RNA molecules. Since the discovery of the first ncRNA in the 1960s, ncRNAs have been shown to be involved in nearly all steps of the central dogma of molecular biology. In recent years, the pace of discovery of novel ncRNAs and their cellular roles has been greatly accelerated by high-throughput sequencing. Advances in sequencing technology, library preparation protocols as well as computational biology helped to greatly expand our knowledge of which ncRNAs exist throughout the kingdoms of life. Moreover, RNA sequencing revealed crucial roles of many ncRNAs in human health and disease. Researchers from the University of Jena discuss the most recent methodological advancements in the rapidly evolving field of high-throughput sequencing and how it has greatly expanded our understanding of ncRNA biology across a large number of different organisms.

Overview of recent ncRNA sequencing techniques 

The scheme summarizes techniques for single-cell total RNA sequencing (RamDA-seq, SMARTer-seq), sequencing of RNAs interacting with DNA by proximity ligation (ChIRP, RAP, CHART, MARGI-seq, GRID-seq, ChAR-seq), interacting in triplexes (TRIP-seq, Sentürk Cetin et al.) and R loops (CUT&RUN, DRIP-seq, MapR, BisMapR) as well as interacting with other RNAs (RIC-seq, MARIO-seq, CLASH, SPLASH).

Micheel J, Safrastyan A, Wollny D. (2021) Advances in Non-Coding RNA Sequencing. Non-Coding RNA [Epub ahead of print]. [abstract]

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