ANAQUIN – a software toolkit for the analysis of spike-in controls for next generation sequencing

Spike-in controls are synthetic nucleic-acid sequences that are added to a user’s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.

rna-seq

Schematic overview of next-generation sequencing workflow, with analytical steps using Anaquin and third-party tools indicated

Availability – The source code is available from https://github.com/student-t/Anaquin, binaries and user manual from www.sequin.xyz/software and R package from http://bioconductor.org/packages/release/bioc/html/Anaquin.html

Wong T, Deveson IW, Hardwick SA, Mercer TR. (2017) ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing. Bioinformatics [Epub ahead of print].[abstract]

Leave a Reply

Your email address will not be published. Required fields are marked *

*

Time limit is exhausted. Please reload CAPTCHA.