Spike-in controls are synthetic nucleic-acid sequences that are added to a user’s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization.

Schematic overview of next-generation sequencing workflow, with analytical steps using Anaquin and third-party tools indicated

Availability – The source code is available from https://github.com/student-t/Anaquin, binaries and user manual from www.sequin.xyz/software and R package from http://bioconductor.org/packages/release/bioc/html/Anaquin.html