Author Archives: RNA-Seq Blog

RVboost: RNA-Seq variants prioritization using a boosting method

rna-seq

RNA-Seq has become the method of choice to quantify genes and exons, discover novel transcripts, and detect fusion genes. However, reliable variant identification from RNA-Seq data remains challenging due to the complexities of the transcriptome, the challenges of accurately mapping ...

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Comprehensive Analysis of Alternative Splicing by Strand-Specific RNA-Seq

rna-seq

Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific ...

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MIRPIPE – quantification of microRNAs in niche model organisms

rna-seq

MicroRNAs represent an important class of small non-coding RNAs regulating gene expression in eukaryotes. Present algorithms typically rely on genomic data to identify miRNAs and require extensive installation procedures. Niche model organisms lacking genomic sequences cannot be analyzed by such ...

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Introduction to RNA-Seq and its Applications to Drug Discovery and Development

rna-seq

The research and development (R&D) process in modern drug discovery and development is a challenging task. One of the reasons is because the biological system is complex. There are 20,000–25,000 genes in the human genome. Using traditional test methods such ...

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RNA sequence could help doctors to tailor unique prostate cancer treatment programs

rna-seq

from Medical Xpress Sequencing RNA, not just DNA, could help doctors predict how prostate cancer tumors will respond to treatment, according to research published in the open access journal Genome Biology. Because a tumor’s RNA shows the real time changes ...

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Establishing Clinical-Grade RNA Sequencing

from Weill Cornell Medical College News The many different approaches used to sequence RNA, even severely degraded RNA, can produce relatively similar results, a finding that should reassure patients, physicians and scientists faced with a variety of new technologies that ...

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NuGEN Introduces Target Enrichment Technology for Comprehensive Detection of Gene Fusion Events in Oncology Research and Clinical Testing

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SAN CARLOS, Calif.–(BUSINESS WIRE)–NuGEN Technologies, Inc., a market leader in the development of solutions for Next Generation Sequencing (NGS) applications, has announced the release of a novel targeted enrichment method that, in a single assay, will detect all possible fusion ...

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Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

rna-seq

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

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The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance

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The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions. Here a team led by researchers atuse at the US FDA comprehensive study design ...

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Qlucore Omics Explorer Makes RNA-Seq Analysis Simple

rna-seq

RNA-seq data can now be analyzed just as easily as microarray data In recent years, transcriptomic profiling via next generation sequencing (RNA-seq) has emerged as both a technical and cost-effective alternative to arrays. Qlucore Omics Explorer 3.0 supports direct import ...

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