Here, scientists at Academia Sinica, Taiwan describe the development of four methods of profiling mRNA 5′ ends using the Illumina sequencing platform; The first method utilizes SMART (Switching Mechanism At 5′ end of RNA Transcript) technology. The second involves replacing ...

This video demonstrates how to use the NIAID HPC Web to do a differential gene expression analysis using Cuffdiff with known annotated genes. Output results are discussed.

ChiloDB is an integrated resource that will be of use to the rice stem borer research community. The rice striped stem borer (SSB), Chilo suppressalis Walker, is a major rice pest that causes severe yield losses in most rice-producing countries. ...

Researchers at Georgia Tech present a new approach to automatic training of a eukaryotic ab initio gene finding algorithm. With the advent of Next-Generation Sequencing, automatic training has become paramount, allowing genome annotation pipelines to keep pace with the speed ...

from Australian Life Scientist by Susan Williamson Hundreds of researchers from 61 institutions have contributed to a collaborative sequence-based ecological study that is attempting to get a handle on the microalgae that populate the sea. Unlike the prokaryotic bacteria, for ...

Despite the prevalent studies of DNA/Chromatin related epigenetics, such as, histone modifications and DNA methylation, RNA epigenetics has not drawn deserved attention until a new affinity-based sequencing approach MeRIP-Seq was developed and applied to survey the global mRNA N6-methyladenosine (m6A) ...

Date: Jul 1, 2014 Location: Indianapolis, IN, US Title: Next Generation Sequencing Scientist- R&D-1402441 Description Dow AgroSciences R&D is seeking an enthusiastic bachelor degree or master degree level Scientist to work in a Next Generation Sequencing (NGS) facility to generate ...

The function of a newly sequenced gene can be discovered by determining its sequence homology with known proteins. BLAST is the most extensively used sequence analysis program for sequence similarity search in large databases of sequences. With the advent of ...

Broad Paper Vids: Single-cell RNA-seq reveals dynamic paracrine control of cellular variation About Broad Paper Vids – Broad Paper Vid is a collection of video abstracts covering Broad scientists’ published papers. In each video, Broad researchers describe the exciting scientific ...

A guided tour of the Exosome RNA-Seq Analysis Kit – Barbara Ruef – Senior Applications Specialist