The production of DNA libraries in sufficient quantity and quality is not only the actual sequencing of the essential step in order to achieve meaningful results in accordance with Next Generation Sequencing (NGS ) process . Automated methods must be ...
Read More »Hobbes2 – for fast and accurate alignment of NGS reads
Next-generation sequencing (NGS) enables rapid production of billions of bases at a relatively low cost. Mapping reads from next-generation sequencers to a given reference genome is an important first step in many sequencing applications. Popular read mappers, such as Bowtie ...
Read More »RNA sequencing of 750-year-old barley virus sheds new light on the Crusades
Scientists have for the first time sequenced an ancient RNA genome – of a barley virus once believed to be only 150 years old – pushing its origin back at least 2,000 years and revealing how intense farming at the ...
Read More »Molecular Med Tri-Con – Genome and Transcriptome Analysis
Genome and Transcriptome Analysis Next-Generation Sequencing of Disease Genomes, Epigenomes & Transcriptomes February 10-12, 2014 | Moscone North Convention Center | San Francisco, CA Over a decade following the completion of the Human Genome Project, next-generation sequencing has progressed from ...
Read More »Library construction for next-generation sequencing: Overviews and challenges
High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, researchers from The Scripps Research Institute ...
Read More »PNAS Publication Demonstrates Power of Molecular Indexing™ Technology for Tracking RNA Molecules in RNA-Seq Library Preparation
PALO ALTO, Calif., Feb. 6, 2014 /PRNewswire/ — Cellular Research, Inc., today announced that its Molecular Indexing™ technology enables the absolute quantification of the starting copy number of an expressed gene, or RNA transcript, in an RNA sequencing (RNA-Seq) library. Data ...
Read More »The MOuse NOnCode Lung database
The MOuse NOnCode Lung database: mouse long non-coding RNAs (lncRNA) involved in Influenza and SARS-CoV infections The MOuse NOnCode Lung database — MONOCLdb — is an integrative and interactive database designed to retrieve and visualize annotations, expression profiles and functional ...
Read More »Advances in NGS & Big Data
SELECTBIO is pleased to present the 4th annual Advances in Next-Gen Sequencing & Big Data conference, which will take place at the Fira Barcelona, on May 14th & 15th 2014. Featuring an array of leading international speakers, this event aims ...
Read More »Identification of novel point mutations in splicing sites integrating whole-exome and RNA-seq data
Point mutations in intronic regions near mRNA splice junctions can affect the splicing process. To identify novel splicing variants from exome sequencing data, researchers at University of Milano–Bicocca, Italy developed a bioinformatics splice-site prediction procedure to analyze next-generation sequencing (NGS) ...
Read More »Transcriptomic Analysis of Endangered Chinese Salamander
The Chinese salamander (Hynobius chinensis), an endangered amphibian species of salamander endemic to China, has attracted much attention because of its value of studying paleontology evolutionary history and decreasing population size. Despite increasing interest in the Hynobius chinensis genome, genomic ...
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