AutoImpute – Autoencoder based imputation of single-cell RNA-seq data

The emergence of single-cell RNA sequencing (scRNA-seq) technologies has enabled us to measure the expression levels of thousands of genes at single-cell resolution. However, insufficient quantities of starting RNA in the individual cells cause significant dropout events, introducing a large number of zero counts in the expression matrix. To circumvent this, researchers from the Indraprastha Institute of IT developed an autoencoder-based sparse gene expression matrix imputation method. AutoImpute, which learns the inherent distribution of the input scRNA-seq data and imputes the missing values accordingly with minimal modification to the biologically silent genes. When tested on real scRNA-seq datasets, AutoImpute performed competitively wrt., the existing single-cell imputation methods, on the grounds of expression recovery from subsampled data, cell-clustering accuracy, variance stabilization and cell-type separability.

AutoImpute pipeline

rna-seq

The raw gene expression data is filtered for bad genes, normalized by library size, pruned by gene-selection and log transformed. Then, the processed matrix is fed to the AutoImpute model for learning expression data representation and finally reconstructing the imputed matrix.

Talwar D, Mongia A, Sengupta D, Majumdar A. (2018) AutoImpute: Autoencoder based imputation of single-cell RNA-seq data. Sci Rep 8(1):16329. [article]

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