from GenomeWeb by Andrew P. Han
NEW YORK (GenomeWeb) – Scientists interested but not well versed in transcriptome profiling can now go to the Broad Institute for help. The Broad Technology Labs Specialized Services Facility offers automated services for high-throughput characterization of both single cells and populations of cells.
Both services are based on high-throughput 3′ digital gene expression protocols Tarjei Mikkelson, a scientist at the Broad Technology Labs, told GenomeWeb. “It’s based on an old concept that you can measure gene expression by sequencing tags or small fragments from each transcript in a cell to measure expression levels of genes,” he said, noting the contrast to RNA-seq methods where the goal is to sequence an entire transcriptome or to get splicing info.