Commentary

Reducing Small RNA Sequencing Biases

rna-seq

from Genetic Engineering News by Adam R. Morris & Masoud M. Toloue Randomized Adapter Strategy for Library Preparation Reduces Ligation Bias and Increases Accuracy of Small RNA-Seq The study of small RNAs, including miRNAs, siRNAs, and pi-RNAs, is an ideal ...

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Are Your RNA-Seq Data Replicable?

rna-seq

from “Big data studies come with replication challenges” by Tina Hesman Saey It has taken more than 15 years for microarray technology to develop enough so that scientists can easily compare their data. Researchers conducting other types of big data ...

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Gene-Editing Guide

rna-seq

from Harvard Univeristy by By SUE McGREEVEY New method identifies genome-wide off-target effects of CRISPR-Cas Harvard Medical School investigators at Massachusetts General Hospital have developed a method for detecting unwanted DNA breaks—across the entire genome of human cells—induced by the ...

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Are we sequencing too deep?

rna-seq

RNA-seq is increasingly used to study gene expression of various organisms. While it provides a great opportunity to explore genome-scale transcriptional patterns with tremendous depth, it comes with prohibitive costs. Establishing a minimal sequencing depth for required accuracy will guide ...

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