Commentary

Researchers at the University of Michigan Comprehensive Cancer Center analyzed the global landscape of a portion of the genome that has not been previously well-explored – long non-coding RNAs. This vast portion of the human genome has been considered the ...

When the human genome was first sequenced, experts predicted they would find about 100,000 genes. The actual number has turned out to be closer to 20,000, just a few thousand more than fruit flies have. The question logically arose: how ...

from University of Chicago, Science Life by Kevin Jiang The majority of RNA expression differences between individuals have no connection to the abundance of a corresponding protein, report scientists from the University of Chicago and Stanford University in Science on ...

A new review article offers a nice overview of attempts to characterize the transcriptome of human stem cells using RNA-seq, the Iso-Seq™ method, and more. Kin Fai Au and Vittorio Sebastiano, scientists at the University of Iowa and Stanford University, ...

from ars technica by Diana Gitig Researchers identify a few cases where twins’ genes behave differently. They’re called identical twins because their genomes are identical. But even though all of their DNA is the same, they clearly are not. The ...

from Harvard Univeristy by By SUE McGREEVEY New method identifies genome-wide off-target effects of CRISPR-Cas Harvard Medical School investigators at Massachusetts General Hospital have developed a method for detecting unwanted DNA breaks—across the entire genome of human cells—induced by the ...

RNA-seq is increasingly used to study gene expression of various organisms. While it provides a great opportunity to explore genome-scale transcriptional patterns with tremendous depth, it comes with prohibitive costs. Establishing a minimal sequencing depth for required accuracy will guide ...

The “RNA world” is not at all hypothetical but rather the biological world we live in (1). For RNA to function within a modern cellular milieu of proteins and DNA, numerous chemical modifications coevolved that help sculpt its interactions (2). ...

Yes, many are attracted to next-generation sequencing, but loyalty to chip-based phenotyping has its rewards, particularly in screens of large sample sets. from Genetic Engineering News – by Lisa Heiden The microarray, the legacy technology for measuring gene expression, will ...

from GenomeWeb by Monica Heger NEW YORK (GenomeWeb) – Researchers from Massachusetts General Hospital have described a method behind two clinical cancer assays — a targeted RNA-seq fusion assay and a hotspot mutation assay — each of which the team ...