Just as RNA itself has primary and secondary structure, RNA-Seq analysis has primary, Secondary and even tertiary steps. Which is the right tool at...
Read More »RNA-Seq Blog Downtime
To Our Readers, Thanks so much for your patience with our recent downtime! The RNA-Seq Blog was attacked by a malicious hacker last Friday. Both our host and developer have been working...
Read More »Single-cell Multi-Omics: Marrying RNA Seq to protein expression and more
Multimodal analysis enables the simultaneous measurement of multiple modalities or the integration of data from multiple experiments. For a long time now, the advantages of sequencing mRNA (scRNA – Seq) in individual cells, to understand...
Read More »Poly-A enrichment or rRNA depletion – how library construction influences RNA sequencing data
Both poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses...
Read More »RNA Stabilization In Cells And Tissues
Ribonucleic Acid (RNA) is the major macromolecule essential for converting genetic codes into proteins through structures called ribosomes for performing cellular functions. The major types of RNA are Transfer RNA (tRNA), Messenger RNA (mRNA), and Ribosomal RNA (rRNA). It can ...
Read More »Introduction and Application of Transcriptome Sequencing
By definition, a transcriptome is the entire collection of messenger Ribonucleic Acid molecules contained in a specific cell or tissue type in an organism. It correlates with the range of RNA being transcribed from a genome under investigation at a ...
Read More »Top Benefits of Using the Technique of Single Cell RNA-Seq
The technology of Single-cell RNA sequencing (scRNA-Seq) has enabled robust as well as simple access to the transcriptomes of the single cells. With Single Cell RNA-Seq, getting better insights about the individual cell tissues has become a lot easier. This, ...
Read More »Guidelines for accurate amplicon-based sequencing of SARS-CoV-2
SARS-CoV-2 genotyping has been instrumental to monitor virus evolution and transmission during the pandemic. The reliability of the information extracted from the genotyping efforts depends on a number...
Read More »HMS Initiative for RNA Medicine
The goal of the HMS Initiative for RNA Medicine is to make and translate RNA discoveries into novel therapeutics and diagnostics by fostering a climate of outstanding basic, translational and clinical research with world-leading scientists and...
Read More »Chan Zuckerburg proposes an initative to advance support and development of the open source Salmon and Alevin software for gene expression quantification of single-cell and bulk RNA-seq
Gene expression quantification is a fundamental computational inference problem at the intersection of genomics, machine learning, and biology. The expression levels (abundances) of genes and transcripts...
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