Technology

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the...

With the advancement of technology, we can generate and access large-scale, high dimensional and diverse genomics data, especially through single-cell RNA sequencing (scRNA-seq). However...

About 15% of human cancer cases are attributed to viral infections. To date, virus expression in tumor tissues has been mostly studied by aligning tumor...

mRNA sequencing (RNA-seq) is a powerful tool that uses next-generation sequencing technologies to examine a cell’s transcriptome profile. This technology has a wide range of applications, from discovering novel transcripts and alternative splicing to analysing the transcriptome of samples in ...

A recent publication from researchers at the University of Kentucky explains the importance of identifying and understanding how differences...

Bacteria within the gut continuously adapt their gene expression to environmental conditions that are associated with diet, health, and disease. Noninvasive...

Sequencing costs currently prohibit the application of single-cell mRNA-seq to many biological and clinical analyses. Targeted single-cell mRNA-sequencing reduces sequencing costs by profiling reduced gene sets that capture biological information with a minimal number of genes. Caltech researchers have developed ...

A team of investigators from Texas Heart Institute, Texas Children’s Hospital and Baylor College of Medicine uncovered new insights into the mechanisms underlying the progression of congenital heart...

Most spatial transcriptomics technologies are limited by their resolution, with spot sizes larger than that of a single cell. Although joint analysis with single-cell...

The novel terminator-assisted solid-phase complementary DNA amplification and sequencing (TAS-Seq) method provides high-precision data on gene expression Single-cell RNA sequencing (scRNA-seq) is one of the most important methods to study biological function in cells, but it is limited by potential ...