RNA-Seq Blog Transcriptome Research & Industry News
scRNA-seq has become a widespread technique within the life sciences but analysis of the data has previously required an in-depth understanding of complex bioinformatics techniques...
Read More »Single-cell RNA-Seq analysis made easy
An easy, cost-effective, and scalable method to deplete human ribosomal RNA for RNA-Seq
RNA sequencing (RNA-seq) is a powerful and increasingly prevalent method to characterize and quantify the transcriptome. Ribosomes are extremely abundant, however, and approximately 80% of total RNA is ribosomal RNA (rRNA). Therefore, to detect and quantify less abundant yet biologically ...
Read More »WIND (Workflow for pIRNAs aNd beyonD): a strategy for in-depth analysis of small RNA-seq data
Current bioinformatics workflows for PIWI-interacting RNA (piRNA) analysis focus primarily on germline-derived piRNAs and piRNA-clusters...
Read More »Experimental approaches and computational workflows for systematic mapping and functional interpretation of RNA modifications
In addition to four nucleotides in mRNA modified nucleotides are an essential addition to the standard genetic code in eukaryotes and prokaryotes. Epitranscriptomics has emerged as a new field to study nucleotide modifications in mRNA and examine their impact on ...
Read More »A single–cell type transcriptomics map of human tissues
Single-cell RNA analysis has been integrated with spatial protein profiling to create a single–cell type map of human tissues...
Read More »Cell-analysis technique could combat tuberculosis
A new method that analyzes how individual immune cells react to the bacteria that cause tuberculosis could pave the way for new vaccine strategies against this deadly disease, and provide insights into fighting other infectious diseases around the world. The ...
Read More »Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping’
The in vivo phenotypic profile of T cells reactive to severe acute respiratory syndrome (SARS)-CoV-2 antigens remains poorly understood. Conventional methods to detect antigen-reactive T cells require in...
Read More »Comprehensive clinical DNA and RNA sequencing opens door to the promise of precision medicine
St. Jude Children’s Research Hospital study highlights the power of comprehensive whole genome, whole exome and RNA sequencing to...
Read More »Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore
RNA modifications, such as N6-methyladenosine (m6A), modulate functions of cellular RNA species. However, quantifying differences in RNA...
Read More »A framework to analyze global transcriptomics in the context of survival behaviors against pathogenesis
Communication with bacteria deeply impacts the life history traits of their hosts. Through specific molecules and metabolites, bacteria can promote short- and long-term phenotypic and behavioral...
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