Methods

Circular RNAs are an emerging class of transcript isoforms created by unique back splicing of exons to form a closed covalent circular structure. While...

While the reconstruction of transcripts from a sample of RNA-Seq data is a computationally expensive and complicated task, the detection of splicing events...

BioTuring Inc. has officially launched BioTuring Browser, the software that integrates state-of-the-art analyses for single-cell RNA-seq data, from transcript quantification, batch effect removal to downstream analyses, all delivered in an interactive visualization dashboard. Latest advances in single-cell RNA-seq (scRNA-seq) technologies ...

Sequencing data has become a standard measure of diverse cellular activities. For example, gene expression is accurately measured by RNA sequencing (RNA-Seq) libraries, protein-DNA interactions are...

The neurodegenerative disease ALS causes motor neuron death and paralysis. However, long before the cells die, they lose contact with the muscles as their axons atrophy. Researchers at Karolinska...

Researchers from the Mayo Clinic used single cell RNA-Seq to examine molecular heterogeneity in multiple myeloma (MM) in 597 CD138 positive cells from bone marrow aspirates of 15 patients at different stages of disease progression. 790 genes were...

In recent years, single-cell RNA sequencing enables us to discover cell types or even subtypes. Its increasing availability provides opportunities to identify cell populations from single-cell RNA-seq data. Computational methods have been employed to...

Researchers from the University of Cambridge have developed a new method to capture protein-RNA complexes which is compatible with downstream proteomics or RNA-Seq. The new method is based on the acid guanidinium thiocyanate-phenol-chloroform (AGPC) which is commonly used to extract ...

Mammalian gene expression is inherently stochastic, and results in discrete bursts of RNA molecules that are synthesized from each allele. Although transcription is known to be regulated by promoters and enhancers, it is unclear how cis-regulatory sequences encode transcriptional burst ...

The advances in high-throughput sequencing technologies are allowing more and more de novo assembling of transcriptomes from many new organisms. Some degree of automation and evaluation is...