Researchers discourage sample pooling in RNA-Seq experiments


Massively parallel cDNA sequencing (RNA-seq) experiments are gradually superseding microarrays in quantitative gene expression profiling. However, many biologists are uncertain about the validity of cost-saving sample pooling strategies for their RNA-seq experiments. Researchers from Aarhus University sequenced RNA-pools and compared their ...

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Assessing technical performance in differential gene expression experiments with external spike-in RNA control ratio mixtures

There is a critical need for standard approaches to assess, report and compare the technical performance of genome-scale differential gene expression experiments. Here, an international team led by researchers at the NIST assess technical performance with a proposed standard ‘dashboard’ ...

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