Data Analysis

RNA sequencing (RNA-seq) is a common and widespread biological assay, and an increasing amount of data is generated with it. In practice, there are a large number of individual steps a researcher must perform before raw RNA-seq reads yield directly ...

Trips-Viz is an interactive platform for the analysis and visualization of ribosome profiling (Ribo-Seq) and shotgun RNA sequencing (RNA-seq) data...

RNA sequencing analysis focus on the detection of differential gene expression changes that meet a two-fold minimum change between groups. The variability present in RNA sequencing data may obscure...

Single-cell RNA sequencing reveals 40 different cell types in Stylophora pistillata, a reef-building stony coral native to the Indo-Pacific oceans. The calcium carbonate skeleton of stony coral colonies are the...

Despite substantial progress in single-cell RNA-seq (scRNA-seq) data analysis methods, there is still little agreement on how to best normalize such data...

Alternative RNA isoforms are defined by promoter choice, alternative splicing, and polyA site selection. Although differential isoform expression is known to play a large regulatory role in eukaryotes, it has proved challenging to study with standard short-read RNA-seq because of ...

A simplistic understanding of the central dogma falls short in correlating the number of genes in the genome to the number of proteins in the proteome.

Next-generation sequencing allows researchers to efficiently determine the sequences of hundreds of millions of short DNA fragments from an experiment. Many experiments use next-generation sequencing...

Analyzing all features of small non-coding RNA sequencing data can be demanding and challenging. To facilitate this process, Saarland University researchers developed miRMaster. After the analysis of over 125 000 human samples and 1.5 trillion...

Single-cell RNA-sequencing (scRNA-Seq) is widely used to reveal the heterogeneity and dynamics of tissues, organisms, and complex diseases, but its analyses still suffer from multiple grand challenges, including the sequencing sparsity and complex...