Analysis Pipelines

Recent single-cell RNA-seq protocols based on droplet microfluidics use massively multiplexed barcoding to enable simultaneous measurements of transcriptomes for thousands of individual cells. The increasing complexity of such data creates challenges for subsequent computational processing...

The quality of RNA sequencing data relies on specific priming by the primer used for reverse transcription (RT-primer). Non-specific annealing of the RT-primer to the RNA template can generate reads with incorrect cDNA ends and can cause misinterpretation...

As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the number of tools designed to analyse these data has dramatically...

Allele-specific transcriptional regulation, including of imprinted genes, is essential for normal mammalian development. While the regulatory regions controlling imprinted genes are associated with DNA methylation (DNAme) and specific histone...

With the advent of the age of big data in bioinformatics, large volumes of data and high performance computing power enable researchers to perform re-analyses of publicly available datasets...

Owing to the advent of high throughput single cell transcriptomics, past few years have seen exponential growth in production of gene expression data. Recently efforts have been made by various research groups to homogenize and store single cell expression from ...

Single cell RNA-seq (scRNA-seq) experiments typically analyze hundreds or thousands of cells after amplification of the cDNA. The high throughput is made possible by the early introduction of sample-specific barcodes (BCs) and the amplification...

RNA sequencing has become a ubiquitous technology used throughout life sciences as an effective method of measuring RNA abundance quantitatively in tissues and cells. The increase in use of RNA-seq technology has led to the continuous development of new tools ...

Version3.0 is now available for download !! Download CSBB-v3.0 from GitHub or Source Forge : GitHub : https://github.com/csbbcompbio/CSBB-v3.0 Source Forge: https://sourceforge.net/projects/csbb-v3-0/?source=navbar Major Highlights of version 3.0: *RNA-Seq processing for human, mouse, frog and zebrafish *ChIP-Seq processing for human, mouse and frog *ATAC-Seq ...

B cells play a critical role in the immune response by producing antibodies, which display remarkable diversity. Here researchers from the Yerkes National Primate Research Centerdescribe a bioinformatic...