Annotation

NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data

rna-seq

The advent of next-generation RNA sequencing (RNA-seq) has greatly advanced transcriptomic studies, including system-wide identification and quantification of mRNA isoforms under various biological conditions. A number of computational methods have been developed to systematically identify mRNA isoforms in a high-throughput ...

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FRAMA – from RNA-seq data to annotated mRNA assemblies

rna-seq

Advances in second-generation sequencing of RNA made a near-complete characterization of transcriptomes affordable. However, the reconstruction of full-length mRNAs via de novo RNA-seq assembly is still difficult due to the complexity of eukaryote transcriptomes with highly similar paralogs and multiple ...

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