Data Normalization

Normalization of RNA-Seq Data Exercises

rna-seq

from RPubs – by Prasanth A S In order to explain the different methods of normalization and their problems with large array of data, which are assumed to have: most genes are not differentially expressed across conditions. the distribution of ...

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RPKM, FPKM and TPM, clearly explained

rna-seq

from StatQuest It used to be when you did RNA-seq, you reported your results in RPKM (Reads Per Kilobase Million) or FPKM (Fragments Per Kilobase Million). However, TPM (Transcripts Per Kilobase Million) is now becoming quite popular. Since there seems ...

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An iteration normalization and test method for differential expression analysis of RNA-seq data

rna-seq

Next generation sequencing technologies are powerful new tools for investigating a wide range of biological and medical questions. Statistical and computational methods are key to analyzing massive and complex sequencing data. In order to derive gene expression measures and compare ...

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