Expression and Quantification

Spatial transcriptomics – quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections

rna-seq

RNA-seq and similar methods can record gene expression within and among cells. Current methods typically lose positional information and many require arduous single-cell isolation and sequencing. A collaboration between professors Jonas Frisén (Karolinska Institutet) and Joakim Lundeberg (Royal Institute of ...

Read More »

Can differential expression methods developed originally for conventional bulk RNA-seq data also be applied to single-cell RNA-seq?

rna-seq

University of Turku researchers compared five statistical methods to detect differentially expressed genes between two distinct single-cell populations. Currently, it remains unclear whether differential expression methods developed originally for conventional bulk RNA-seq data can also be applied to single-cell RNA-seq ...

Read More »

multiDE – a dimension reduced model based statistical method for differential expression analysis using RNA-sequencing data with multiple treatment conditions

rna-seq

The growing complexity of biological experiment design based on high-throughput RNA sequencing (RNA-seq) is calling for more accommodative statistical tools. Researchers at Fudan University have developed a novel method, multiDE, for facilitating DE analysis using RNA-seq read count data with ...

Read More »