Expression and Quantification

You’ve been doing your RNA-Seq all wrong


In recent years, RNA-seq is emerging as a powerful technology in estimation of gene and/or transcript expression, and RPKM (Reads Per Kilobase per Million reads) is widely used to represent the relative abundance of mRNAs for a gene. In general, ...

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Evaluation of data discretization methods to derive platform independent isoform expression signatures for multi-class tumor subtyping

Many supervised learning algorithms have been applied in deriving gene signatures for patient stratification from gene expression data. However, transferring the multi-gene signatures from one analytical platform to another without loss of classification accuracy is a major challenge. Here, researchers ...

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