RNA modifications, such as N6-methyladenosine (m6A), modulate functions of cellular RNA species. However, quantifying differences in RNA...
Read More »Optimizing expression quantitative trait locus mapping workflows for single-cell studies
Single-cell RNA sequencing (scRNA-seq) has enabled the unbiased, high-throughput quantification of gene expression specific to cell types and states. With...
Read More »SCENA – Consensus clustering of single-cell RNA-seq data by enhancing network affinity
Elucidation of cell subpopulations at high resolution is a key and challenging goal of single-cell ribonucleic acid (RNA) sequencing (scRNA-seq)...
Read More »Normalized counts performs better than TPM, FPKM for hierarchical clustering of replicate RNA-Seq samples
In order to correctly decode phenotypic information from RNA-sequencing (RNA-seq) data, careful selection of the RNA-seq quantification measure is critical for inter-sample comparisons and for...
Read More »GeneTonic – Enjoy your transcriptomic data analysis with streamlined interpretation
Computational workflows for RNA sequencing data often include differential expression and functional interpretation analyses, and a number of specialized and established tools exist for performing these tasks, each generating a set of intermediate processed data and results. In order to ...
Read More »A clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations
The ability to discover new cell populations by unsupervised clustering of single-cell transcriptomics data has revolutionized biology. However, all unsupervised methods have adjustable parameters, which renders it difficult for researchers to decide on the right resolution for clustering. Often it ...
Read More »SPEAQeasy – a scalable pipeline for expression analysis and quantification for R/bioconductor-powered RNA-seq analyses
RNA sequencing (RNA-seq) is a common and widespread biological assay, and an increasing amount of data is generated with it. In practice, there are a large number of individual steps a researcher must perform before raw RNA-seq reads yield directly ...
Read More »Sanity – bayesian inference of gene expression states from single-cell RNA-seq data
Despite substantial progress in single-cell RNA-seq (scRNA-seq) data analysis methods, there is still little agreement on how to best normalize such data...
Read More »LR-Split-seq – mapping and modeling the genomic basis of differential RNA isoform expression at single-cell resolution
Alternative RNA isoforms are defined by promoter choice, alternative splicing, and polyA site selection. Although differential isoform expression is known to play a large regulatory role in eukaryotes, it has proved challenging to study with standard short-read RNA-seq because of ...
Read More »Model-based deep embedding for constrained clustering analysis of single cell RNA-seq data
Clustering is a critical step in single cell-based studies. Most existing methods support unsupervised clustering without the a priori exploitation of any domain knowledge. When confronted by the high dimensionality and pervasive dropout events...
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