Expression and Quantification

A team led by researchers at the Memorial Sloan-Kettering Cancer Center present Oqtans, an open-source workbench for quantitative transcriptome analysis, that is integrated in the Galaxy framework. Its distinguishing features include customizable computational workflows and a modular pipeline architecture that ...

In the last decade, Next-Generation Sequencing technologies have been extensively applied to quantitative transcriptomics, making RNA sequencing a valuable alternative to microarrays for measuring and comparing gene transcription levels. Although several methods have been proposed to provide an unbiased estimate ...

RNA-sequencing (RNA-seq) is a flexible technology for measuring genome-wide expression that is rapidly replacing microarrays as costs become comparable. Current differential expression analysis methods for RNA-seq data fall into two broad classes: (1) methods that quantify expression within the boundaries ...

RNA sequencing (RNA-Seq) part 2: expression level estimation and differential expression analysis Vincent Plagnol UCL Genetics Institute