The sequencing, de novo assembly and annotation of transcriptome datasets generated with next generation sequencing (NGS) has enabled biologists to answer genomic questions in non-model species with unprecedented ease. Reliable and accurate de novo assembly and annotation of transcriptomes, however, ...
Read More »bagSVM – Classification of RNA-Seq Data via Bagging Support Vector Machines
RNA sequencing (RNA-Seq) is a powerful technique for transcriptome profiling of the organisms that uses the capabilities of next-generation sequencing (NGS) technologies. Recent advances in NGS let to measure the expression levels of tens to thousands of transcripts simultaneously. Using ...
Read More »JAGuaR: Junction Alignments to Genome for RNA-Seq Reads
JAGuaR is an alignment protocol for RNA-seq reads that uses an extended reference to increase alignment sensitivity. It uses BWA to align reads to the genome and reference transcript models (including annotated exon-exon junctions) specifically allowing for the possibility of ...
Read More »iRegulon: From a Gene List to a Gene Regulatory Network
Identifying master regulators of biological processes and mapping their downstream gene networks are key challenges in systems biology. Researchers at the KU Leuven Center for Human Genetics developed a computational method, called iRegulon, to reverse-engineer the transcriptional regulatory network underlying ...
Read More »Researchers at the University of Liverpool are building the world’s most comprehensive database
Researchers at the University of Liverpool are building the world’s most comprehensive database describing human and animal pathogens, which can be used to prevent and tackle disease outbreaks around the globe. The Enhanced Infectious Diseases (EID2) database has been developed ...
Read More »SpliceNet: recovering splicing isoform-specific differential gene networks from RNA-Seq data of normal and diseased samples
Conventionally, overall gene expressions from microarrays are used to infer gene networks, but it is challenging to account splicing isoforms. High-throughput RNA Sequencing has made splice variant profiling practical. However, its true merit in quantifying splicing isoforms and isoform-specific exon ...
Read More »STATegra EMS: an Experiment Management System for complex next-generation omics experiments
High-throughput sequencing assays are now routinely used to study different aspects of genome organization. As decreasing costs and widespread availability of sequencing enable more laboratories to use sequencing assays in their research projects, the number of samples and replicates in ...
Read More »MAMBA – Assessing allele specific expression across multiple tissues from RNA-seq read data
RNA sequencing enables allele specific expression (ASE) studies that complement standard genotype expression studies for common variants and, importantly, also allow measuring the regulatory impact of rare variants. The Genotype-Tissue Expression project (GTEx) is collecting RNA-seq data on multiple tissues ...
Read More »SigFuge – single gene clustering of RNA-seq reveals differential isoform
High-throughput sequencing technologies, including RNA-seq, have made it possible to move beyond gene expression analysis to study transcriptional events including alternative splicing and gene fusions. Furthermore, recent studies in cancer have suggested the importance of identifying transcriptionally altered loci as ...
Read More »Incorporating RNA-Seq data to improve sensitivity of protein identification by tandem MS
Tandem mass spectrometry (MS/MS) followed by database search is the method of choice for protein identification in proteomic studies. Database searching methods employ spectral matching algorithms and statistical models to identify and quantify proteins in a sample. In general, these ...
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