Splicing and Junction Mapping

Designing alternative splicing RNA-seq studies

rna-seq

D esigning an RNA-seq study depends critically on its specific goals, technology and underlying biology, which renders general guidelines inadequate.  A team led by researchers at IRB Barcelona have developed a Bayesian framework to customize experiments so that goals can ...

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Comprehensive transcriptome analysis using synthetic long-read sequencing reveals molecular co-association of distant splicing events

rna-seq

Alternative splicing shapes mammalian transcriptomes, with many RNA molecules undergoing multiple distant alternative splicing events. Comprehensive transcriptome analysis, including analysis of exon co-association in the same molecule, requires deep, long-read sequencing. Here we introduce an RNA sequencing method, synthetic long-read ...

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