The main critical step in single-cell transcriptomics is sample preparation. Several methods have been developed to preserve cells after dissociation to...
Read More »Blocking abundant RNA transcripts by high-affinity oligonucleotides during transcriptome library preparation
RNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of low-abundant transcripts. In contrast to microarray technology, RNA...
Read More »A novel method for for cell fixation before dissociation and single-cell sequencing
Single-cell RNA-sequencing (scRNA-seq) presents an opportunity to deconstruct cellular networks but is limited by the loss of biological information, including in vivo cellular states and phospho-signaling. Researchers from the University of Alabama at...
Read More »SHERRY2 – a specifically optimized protocol for scRNA-seq without second strand cDNA synthesis
Prevalent single cell transcriptomic profiling (scRNA-seq) mechods are mainly based on synthesis and enrichment of full-length double-stranded complementary DNA. These approaches are challenging to generate accurate quantification of transcripts...
Read More »Improving an rRNA depletion protocol with statistical design of experiments
In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so...
Read More »RiboRid – A low cost, advanced, and ultra-efficient method to remove ribosomal RNA for bacterial transcriptomics
RNA sequencing techniques have enabled the systematic elucidation of gene expression (RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq), and post...
Read More »CircAID-p-seq – a PCR-free library preparation for selective 3′ phospho-RNA sequencing
Most RNA footprinting approaches that require ribonuclease cleavage generate RNA fragments bearing a phosphate or cyclic phosphate group...
Read More »Phospho-RNAseq – profiling of extracellular mRNAs and lncRNAs
Extracellular RNAs (exRNAs) in biofluids have attracted great interest as potential biomarkers. Although extracellular microRNAs in blood plasma are extensively characterized, extracellular...
Read More »ulRNA-seq – a library construction scheme for high sensitivity and low abundance gene detection in subcellular or ultralow RNA sequencing
Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input...
Read More »Ordered two-template relay for automation-friendly, low-bias, end-to-end RNA sequence inventories of complex ncRNA samples
NGS libraries from sRNA are commonly prepared by sequential ligation of adaptors to input RNA 3′ and 5′ ends, often with a gel purification step after each ligation step, followed by cDNA synthesis and PCR...
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