Prevalent single cell transcriptomic profiling (scRNA-seq) mechods are mainly based on synthesis and enrichment of full-length double-stranded complementary DNA. These approaches are challenging to generate accurate quantification of transcripts...
Read More »Improving an rRNA depletion protocol with statistical design of experiments
In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so...
Read More »RiboRid – A low cost, advanced, and ultra-efficient method to remove ribosomal RNA for bacterial transcriptomics
RNA sequencing techniques have enabled the systematic elucidation of gene expression (RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq), and post...
Read More »CircAID-p-seq – a PCR-free library preparation for selective 3′ phospho-RNA sequencing
Most RNA footprinting approaches that require ribonuclease cleavage generate RNA fragments bearing a phosphate or cyclic phosphate group...
Read More »Phospho-RNAseq – profiling of extracellular mRNAs and lncRNAs
Extracellular RNAs (exRNAs) in biofluids have attracted great interest as potential biomarkers. Although extracellular microRNAs in blood plasma are extensively characterized, extracellular...
Read More »ulRNA-seq – a library construction scheme for high sensitivity and low abundance gene detection in subcellular or ultralow RNA sequencing
Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input...
Read More »Ordered two-template relay for automation-friendly, low-bias, end-to-end RNA sequence inventories of complex ncRNA samples
NGS libraries from sRNA are commonly prepared by sequential ligation of adaptors to input RNA 3′ and 5′ ends, often with a gel purification step after each ligation step, followed by cDNA synthesis and PCR...
Read More »An easy, cost-effective, and scalable method to deplete human ribosomal RNA for RNA-Seq
RNA sequencing (RNA-seq) is a powerful and increasingly prevalent method to characterize and quantify the transcriptome. Ribosomes are...
Read More »New method to add “Time” resolution to your single-cell RNA-seq
The understanding of individual cells has enormously improved with the application of single-cell RNA-sequencing approaches, enabling researchers to...
Read More »IsoSeek – unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution
Terminal nucleotidyl transferases are enzymes that add non-templated nucleotides to RNA molecules. In the case of microRNAs, this process was shown to be functionally relevant for their maturation process...
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