RNA-Seq Blog Transcriptome Research & Industry News
Most RNA footprinting approaches that require ribonuclease cleavage generate RNA fragments bearing a phosphate or cyclic phosphate group...
Read More »CircAID-p-seq – a PCR-free library preparation for selective 3′ phospho-RNA sequencing
Phospho-RNAseq – profiling of extracellular mRNAs and lncRNAs
Extracellular RNAs (exRNAs) in biofluids have attracted great interest as potential biomarkers. Although extracellular microRNAs in blood plasma are extensively characterized, extracellular...
Read More »ulRNA-seq – a library construction scheme for high sensitivity and low abundance gene detection in subcellular or ultralow RNA sequencing
Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input...
Read More »Ordered two-template relay for automation-friendly, low-bias, end-to-end RNA sequence inventories of complex ncRNA samples
NGS libraries from sRNA are commonly prepared by sequential ligation of adaptors to input RNA 3′ and 5′ ends, often with a gel purification step after each ligation step, followed by cDNA synthesis and PCR...
Read More »An easy, cost-effective, and scalable method to deplete human ribosomal RNA for RNA-Seq
RNA sequencing (RNA-seq) is a powerful and increasingly prevalent method to characterize and quantify the transcriptome. Ribosomes are...
Read More »New method to add “Time” resolution to your single-cell RNA-seq
The understanding of individual cells has enormously improved with the application of single-cell RNA-sequencing approaches, enabling researchers to...
Read More »IsoSeek – unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution
Terminal nucleotidyl transferases are enzymes that add non-templated nucleotides to RNA molecules. In the case of microRNAs, this process was shown to be functionally relevant for their maturation process...
Read More »Ribo-ODDR – Oligo design pipeline for experiment-specific rRNA depletion in ribo-seq
Ribosome Profiling (Ribo-seq) has revolutionized the study of RNA translation by providing information on ribosome positions across all translated RNAs with...
Read More »High-throughput minitaturized RNA-Seq library preparation
Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research...
Read More »Monodisperse drops templated by 3D-structured microparticles – a foundation for sensitive compartmentalized molecular and cell-based assays with minimal instrumentation
The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has...
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