Library Preparation

Prevalent single cell transcriptomic profiling (scRNA-seq) mechods are mainly based on synthesis and enrichment of full-length double-stranded complementary DNA. These approaches are challenging to generate accurate quantification of transcripts...

In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so...

RNA sequencing techniques have enabled the systematic elucidation of gene expression (RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq), and post...

Most RNA footprinting approaches that require ribonuclease cleavage generate RNA fragments bearing a phosphate or cyclic phosphate group...

Extracellular RNAs (exRNAs) in biofluids have attracted great interest as potential biomarkers. Although extracellular microRNAs in blood plasma are extensively characterized, extracellular...

Single-cell RNA sequencing (scRNA-seq) provides new insights to address biological and medical questions, and it will benefit more from the ultralow input...

NGS libraries from sRNA are commonly prepared by sequential ligation of adaptors to input RNA 3′ and 5′ ends, often with a gel purification step after each ligation step, followed by cDNA synthesis and PCR...

RNA sequencing (RNA-seq) is a powerful and increasingly prevalent method to characterize and quantify the transcriptome. Ribosomes are...

The understanding of individual cells has enormously improved with the application of single-cell RNA-sequencing approaches, enabling researchers to...

Terminal nucleotidyl transferases are enzymes that add non-templated nucleotides to RNA molecules. In the case of microRNAs, this process was shown to be functionally relevant for their maturation process...