Library Preparation

Dr. Michaela Bowden of the Dana Farber Cancer Institute describes automation of RNA-Seq of archival samples for high throughput on a Beckman FX. Automation of RNA-Seq improves library quality consistency among difficult (FFPE) samples and enables higher throughput

MicroRNAs play an important role in regulation and disease progression, and thus are increasingly being characterized by next-generation sequencing (NGS). One of the challenges in deep sequencing of the small RNAs (small RNA-Seq) is in the sample preparation workflow. This ...

RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA ...

A major hurdle to transcriptome profiling by deep-sequencing technologies is that abundant transcripts, such as rRNAs, can overwhelm the libraries, severely reducing transcriptome-wide coverage. Methods for depletion of such unwanted sequences typically require treatment of RNA samples prior to library ...

Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting ...

~Improved RNA-Seq data quality and lower sequencing costs enabled April 24, 2014 06:00 AM Eastern Daylight Time SAN CARLOS, Calif.–(BUSINESS WIRE)–NuGEN Technologies, Inc., a market leader in the development of sample preparation solutions for Next Generation Sequencing (NGS) applications, has ...

The mammalian lung is a highly branched network in which the distal regions of the bronchial tree transform during development into a densely packed honeycomb of alveolar air sacs that mediate gas exchange. Although this transformation has been studied by ...

This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...

RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages ...

from Cofactor Genomics 1. Sample Quality and and stringent QC measures First and foremost, if a sample is of low quality or complexity, both of which can occur due to degradation, then it is best to either not sequence or ...