Library Preparation

Official release of TeloPrime Full-Length cDNA Amplification Kit

rna-seq

TeloPrime enables the amplification of full-length cDNA with extreme stringency based on a new innovative technology. It allows more faithful tagging of full-length RNA transcripts that are both capped and polyadenylated compared to any other protocol available. The new technology ...

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Bioo Scientific First to Address Small RNA-Seq Bias Concerns with Launch of New Kit

rna-seq

Author: BIOO/Monday, August 11, 2014 Bioo Scientific launched the patent pending NEXTflex™ Small RNA Sequencing Kit v2, which utilizes randomized adapters to significantly reduce ligation bias from library preparation, resulting in the most accurate data available, as illustrated in a ...

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Success in RNA-Seq – Sample Prep and Workflow considerations

rna-seq

by Natalie McDonald, PhD RNA in the cell is comprised of a variety of populations, including traditional poly-adenylated messenger RNA (polyA RNA), non-adenlyated RNA, which may be coding or regulatory, ribosomal RNA (rRNA) and small and micro RNA.  The first ...

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An efficient and sensitive method for preparing cDNA libraries from scarce biological samples

rna-seq

The preparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful tool to quantify known small RNAs (such as microRNAs) and to discover novel RNA species. Interest in identifying the small RNA repertoire present in ...

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Microfluidic single-cell whole-transcriptome sequencing

rna-seq

Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting ...

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