Dr. Michaela Bowden of the Dana Farber Cancer Institute describes automation of RNA-Seq of archival samples for high throughput on a Beckman FX. Automation of RNA-Seq improves library quality consistency among difficult (FFPE) samples and enables higher throughput
Read More »Improved Small RNA Library Preparation Workflows for Next Generation Sequencing
MicroRNAs play an important role in regulation and disease progression, and thus are increasingly being characterized by next-generation sequencing (NGS). One of the challenges in deep sequencing of the small RNAs (small RNA-Seq) is in the sample preparation workflow. This ...
Read More »Comparison of RNA-Seq by poly (A) capture, ribosomal RNA depletion, and DNA microarray for expression profiling
RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA ...
Read More »Selective and flexible depletion of problematic sequences from RNA-seq libraries at the cDNA stage
A major hurdle to transcriptome profiling by deep-sequencing technologies is that abundant transcripts, such as rRNAs, can overwhelm the libraries, severely reducing transcriptome-wide coverage. Methods for depletion of such unwanted sequences typically require treatment of RNA samples prior to library ...
Read More »Microfluidic single-cell whole-transcriptome sequencing
Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting ...
Read More »Novel Technology for RNA-Seq Extends to a Broad Range of Transcript Species
~Improved RNA-Seq data quality and lower sequencing costs enabled April 24, 2014 06:00 AM Eastern Daylight Time SAN CARLOS, Calif.–(BUSINESS WIRE)–NuGEN Technologies, Inc., a market leader in the development of sample preparation solutions for Next Generation Sequencing (NGS) applications, has ...
Read More »Microfluidic single-cell RNA sequencing (RNA-seq) on 198 individual cells
The mammalian lung is a highly branched network in which the distal regions of the bronchial tree transform during development into a densely packed honeycomb of alveolar air sacs that mediate gas exchange. Although this transformation has been studied by ...
Read More »Next-Generation Sequencing RNA-Seq Library Construction
This unit presents protocols for construction of next-generation sequencing (NGS) directional RNA sequencing libraries for the Illumina HiSeq and MiSeq from a wide variety of input RNA sources. The protocols are based on the New England Biolabs (NEB) small RNA ...
Read More »Quality assessment and control of tissue specific RNA-seq libraries
RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages ...
Read More »6 Changes That’ll Make a Big Difference With Your RNA-seq
from Cofactor Genomics 1. Sample Quality and and stringent QC measures First and foremost, if a sample is of low quality or complexity, both of which can occur due to degradation, then it is best to either not sequence or ...
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