Library Preparation

TeloPrime enables the amplification of full-length cDNA with extreme stringency based on a new innovative technology. It allows more faithful tagging of full-length RNA transcripts that are both capped and polyadenylated compared to any other protocol available. The new technology ...

Author: BIOO/Monday, August 11, 2014 Bioo Scientific launched the patent pending NEXTflex™ Small RNA Sequencing Kit v2, which utilizes randomized adapters to significantly reduce ligation bias from library preparation, resulting in the most accurate data available, as illustrated in a ...

by Natalie McDonald, PhD RNA in the cell is comprised of a variety of populations, including traditional poly-adenylated messenger RNA (polyA RNA), non-adenlyated RNA, which may be coding or regulatory, ribosomal RNA (rRNA) and small and micro RNA.  The first ...

The preparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful tool to quantify known small RNAs (such as microRNAs) and to discover novel RNA species. Interest in identifying the small RNA repertoire present in ...

Here, scientists at Academia Sinica, Taiwan describe the development of four methods of profiling mRNA 5′ ends using the Illumina sequencing platform; The first method utilizes SMART (Switching Mechanism At 5′ end of RNA Transcript) technology. The second involves replacing ...

Dr. Michaela Bowden of the Dana Farber Cancer Institute describes automation of RNA-Seq of archival samples for high throughput on a Beckman FX. Automation of RNA-Seq improves library quality consistency among difficult (FFPE) samples and enables higher throughput

MicroRNAs play an important role in regulation and disease progression, and thus are increasingly being characterized by next-generation sequencing (NGS). One of the challenges in deep sequencing of the small RNAs (small RNA-Seq) is in the sample preparation workflow. This ...

RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA ...

A major hurdle to transcriptome profiling by deep-sequencing technologies is that abundant transcripts, such as rRNAs, can overwhelm the libraries, severely reducing transcriptome-wide coverage. Methods for depletion of such unwanted sequences typically require treatment of RNA samples prior to library ...

Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting ...