Optimizing antibody use for accurate m6A mapping in RNA research
In the ever-evolving field of RNA research, N6-methyladenosine (m6A) has emerged as a key player in regulating gene expression. m6A is a chemical modification on RNA that influences various processes, including RNA stability, translation, and cellular responses. To study ...
Sample multiplexing for retinal single-cell RNA-sequencing
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying gene expression at the cellular level. However, it can be tricky to analyze rare cell populations, especially when working with tiny, hard-to-find groups of cells. Researchers often face the ...
Evaluation of RNA extraction library preparation methods to detect viral RNA from different sample matrices
Human cells, tissues, and cellular and tissue base products (HCT/Ps) are required to comply with the donor eligibility requirements as per 21 CFR part 1271 and applicable guidance documents to prevent the introduction, transmission, and spread of communicable diseases by ...
Exploring the impact of primer length on efficient gene detection via RNA sequencing
Reverse transcription (RT) is a fundamental process in RNA analysis, converting RNA into complementary DNA (cDNA). This step is essential for various...
One-pot method for preparing DNA, RNA, and protein for multiomics analysis
Unraveling the complexities of diseases such as cancer often requires a multi-faceted approach, analyzing various molecular components like DNA...
SLiPiR-seq – terminal modifications independent cell-free RNA sequencing
Cell-free RNAs (cfRNAs) offer an opportunity to detect diseases from a transcriptomic perspective, however, existing techniques have fallen short in generating a comprehensive cell-free transcriptome profile. Researchers at Shenzhen University have developed a sensitive library preparation method that is robust ...
Decoder-seq – dendrimeric DNA coordinate barcoding design for spatial RNA sequencing
Spatially resolved transcriptomic technologies show promise in revealing complex pathophysiological processes, but developing sensitive, high-resolution, and cost-effective methodology is challenging. Researchers at the Shanghai Jiao Tong University School of...
Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells
The main critical step in single-cell transcriptomics is sample preparation. Several methods have been developed to preserve cells after dissociation to...
Blocking abundant RNA transcripts by high-affinity oligonucleotides during transcriptome library preparation
RNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of low-abundant transcripts. In contrast to microarray technology, RNA...
A novel method for for cell fixation before dissociation and single-cell sequencing
Single-cell RNA-sequencing (scRNA-seq) presents an opportunity to deconstruct cellular networks but is limited by the loss of biological information, including in vivo cellular states and phospho-signaling. Researchers from the University of Alabama at...
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