RNA-Seq Blog Transcriptome Research & Industry News
The understanding of individual cells has enormously improved with the application of single-cell RNA-sequencing approaches, enabling researchers to...
Read More »New method to add “Time” resolution to your single-cell RNA-seq
IsoSeek – unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution
Terminal nucleotidyl transferases are enzymes that add non-templated nucleotides to RNA molecules. In the case of microRNAs, this process was shown to be functionally relevant for their maturation process...
Read More »Ribo-ODDR – Oligo design pipeline for experiment-specific rRNA depletion in ribo-seq
Ribosome Profiling (Ribo-seq) has revolutionized the study of RNA translation by providing information on ribosome positions across all translated RNAs with...
Read More »High-throughput minitaturized RNA-Seq library preparation
Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research...
Read More »Monodisperse drops templated by 3D-structured microparticles – a foundation for sensitive compartmentalized molecular and cell-based assays with minimal instrumentation
The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has...
Read More »Targeted single-cell RNA and DNA sequencing with fluorescence-activated droplet merger
Analyzing every cell in a diverse sample provides insight into population-level heterogeneity, but abundant cell types dominate the analysis and...
Read More »Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids
Background Small RNA sequencing (RNA‐seq) of biofluids is challenging due to the relative scarcity of microRNAs (miRNAs), limited sample volumes, and the lack of a gold standard isolation method. Additionally, few comparisons exist for the RNA isolation and sequencing methods ...
Read More »Ribo-Pop – Simple, cost-effective, and widely applicable ribosomal RNA depletion
The measurement of RNA abundance derived from massively parallel sequencing experiments is an essential technique. Methods that reduce ribosomal RNA levels are usually required prior to sequencing library construction because ribosomal RNA typically...
Read More »A low-bias and sensitive small RNA library preparation method using randomized splint ligation
Small RNAs are important regulators of gene expression and are involved in human development and disease. Next generation sequencing (NGS) allows for scalable, genome-wide studies of small RNA; however, current methods are challenged by low...
Read More »Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads
A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative non-ribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal...
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